Human GDF-5/BMP-14 Antibody

Catalog #: MAB8340 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8340-100
MAB8340-SP

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Detection of GDF‑5/BMP‑14 in U-118MG (positive) and MCF-7 (negative) cells.

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Human GDF-5/BMP-14 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GDF-5 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1065032

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GDF-5
Ala382-Arg501
Accession # P43026

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed U-118 MG human glioblastoma/astrocytoma cells (positive) and MCF-7 MG human glioblastoma cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of GDF‑5/BMP‑14 in U-118MG (positive) and MCF-7 (negative) cells. GDF‑5/BMP‑14 was detected in immersion fixed U‑118 MG human glioblastoma/astrocytoma cells (positive) and absent in MCF-7 MG human glioblastoma cells (negative) using Mouse Anti-Human GDF‑5/BMP‑14 Monoclonal Antibody (Catalog # MAB8340) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-5/BMP-14

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1) and BMP-14, is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF‑ beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N‑terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a subgroup within the BMP family. Like other TGF‑ beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References

Storm, E.E. et al. (1994) Nature 368:639.
Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
Francis-West, P.H. et al. (1999) Development 126:1035.
Massague, J. et al. (2000) Genes and Dev. 14:627.
Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
Inada, M. et al. (1996) BBRC 222:317.

Long Name

Growth Differentiation Factor 5

Entrez Gene IDs

8200 (Human); 14563 (Mouse)

Alternate Names

BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2