Human GFR alpha-4/GDNF R alpha-4 Antibody

Catalog # Availability Size / Price Qty
MAB1439
MAB1439-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human GFR alpha-4/GDNF R alpha-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GFR alpha ‑4/GDNF R alpha ‑4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) GFR alpha -1, recombinant rat GFR alpha -1, rhGFR alpha -2, rhGFR alpha -3, or recombinant mouse GFR alpha -4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 215725
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GFR alpha ‑4/GDNF R alpha ‑4
Asn24-Ser245 (Ser140Cys)(predicted)
Accession # NP_071422
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GFR alpha ‑4/GDNF R alpha ‑4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-4/GDNF R alpha-4

GFR alpha -4 is a member of a family of at least four cysteine-rich GPI-linked cell surface proteins that function as ligand binding subunits for the GDNF family (GDNF, neurturin, persephin and artemin). A functional receptor complex is achieved through association with the c-Ret receptor tyrosine kinase. GFR alpha -4 preferentially binds persephin.

Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 4
Entrez Gene IDs
64096 (Human); 14588 (Mouse)
Alternate Names
GDNF family receptor alpha 4; GDNF family receptor alpha-4; GDNF R alpha-4; GDNF receptor alpha-4; GDNFR-alpha-4; GFR alpha4; GFR alpha-4; GFR receptor alpha 4; GFRA4; GFRa-4; GFR-alpha-4; Persephin receptor

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Citations for Human GFR alpha-4/GDNF R alpha-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Expression of Glial-Cell-Line-Derived Neurotrophic Factor Family Ligands in Human Intervertebral Discs
    Authors: Iwasaki, T;Akeda, K;Kawaguchi, K;Yamada, J;Hasegawa, T;Takegami, N;Fujiwara, T;Sudo, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin
    Authors: Frank L. Rice, George Houk, James P. Wymer, Sara J. C. Gosline, Justin Guinney, Jianqiang Wu et al.
    PLOS ONE

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