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Human GFR alpha -like Antibody

Catalog #: MAB9697 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9697-100
MAB9697-SP
Detection of GFR alpha -like in HEK293 Human Cell Line Transfected with Human GFR alpha -like by Flow Cytometry.
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Human GFR alpha -like Antibody Summary

Species Reactivity
Human
Specificity
Detects human GFR alpha ‑like in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2345C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human GFR alpha ‑like
Ser19-Glu351
Accession # Q6UXV0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA binding assay, 100-800 ng/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human GDF-15 (Catalog # 9279-GD) to immobilized Recombinant Human GFR alpha -like Fc Chimera (Catalog # 9697-GR) coated at 0.1 μg/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GFRa-like antibody in HEK293 Human Cell Line Transfected with Human GFRa-like antibody by Flow Cytometry. View Larger

Detection of GFR alpha -like in HEK293 Human Cell Line Transfected with Human GFR alpha -like by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GFRa-like (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Rabbit Anti-Human GFRa-like Monoclonal Antibody (Catalog # MAB9697) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71%  identity with mouse and rat GFRAL respectively. It is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling (2-4). GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

References
  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Mullican, S. et al. (2017) Nat. Med 23:1150.
  3. Yang, L. et al. (2017) Nat. Med 23: 1158.
  4. Emmerson, P. et al (2017) Nat. Med 23:1215.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
Entrez Gene IDs
389400 (Human); 404194 (Mouse); 501023 (Rat)
Alternate Names
bA360D14.1; C6orf144; GDNF family receptor alpha-like; GFR alpha like; GFR alpha-like; GFRAL; GRAL; IVFI9356; UNQ9356

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