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Human GITR/TNFRSF18 Antibody

Catalog #: MAB689
11 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB689-SP
MAB689-100
MAB689-500
Detection of GITR/TNFRSF18 in RPMI8226 (positive cells) vs. Jurkat (negative cells) by Flow Cytometry
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Citations (11)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GITR/TNFRSF18 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse
(rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 110416
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
Gln26-Glu161 (Thr43Ala)
Accession # Q9Y5U5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR)
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
- Human peripheral blood CD4+ T cells treated with PHA

- RPMI8226 cells
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-2 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human GITR Ligand/TNFSF18 (Catalog # 694-GL) to immobilized Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR) coated at 2 µg/mL (100 µL/well). At 5 µg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human GITR/TNFRSF18 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human GITR/TNFRSF18 Biotinylated Antibody (Catalog # BAF689)

Standard: Recombinant Human GITR/TNFRSF18 Fc Chimera Protein, CF (Catalog # 689-GR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of GITR/TNFRSF18 in RPMI8226 (positive cells) vs. Jurkat (negative cells) by Flow Cytometry RPMI8226 cells (filled histogram) vs. Jurkat cells (open histogram) were stained with Mouse Anti-Human GITR/TNFRSF18 Monoclonal Antibody (Catalog # MAB689) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is
up-regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

References
  1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  3. Gurney, A.L. et al. (1999) Current Biology 9:215.
  4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.
Long Name
Glucocorticoid Induced TNF Receptor Family Related Gene
Entrez Gene IDs
8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
Alternate Names
Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18

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Citations for Human GITR/TNFRSF18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. MEDI1873, a potent, stabilized hexameric agonist of human GITR with regulatory T-cell targeting potential
    Authors: Natalie J. Tigue, Lisa Bamber, John Andrews, Samantha Ireland, James Hair, Edward Carter et al.
    OncoImmunology
  2. Predictive biomarkers for the responsiveness of recurrent glioblastomas to activated killer cell immunotherapy
    Authors: S Hwang, J Lim, H Kang, JY Jeong, JG Joung, J Heo, D Jung, K Cho, HJ An
    Cell & bioscience, 2023-01-24;13(1):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Generation and functional characterization of MDSC-like cells
    Authors: A Heine, SAE Held, J Schulte-Sc, JFA Wolff, K Klee, T Ulas, NA Schmacke, SN Daecke, K Riethausen, JL Schultze, P Brossart
    Oncoimmunology, 2017-02-23;6(4):e1295203.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. The effects of CAMPATH-1H on cell viability do not correlate to the CD52 density on the cell surface.
    Authors: Lee F, Luevano M, Veys P, Yong K, Madrigal A, Shaw B, Saudemont A
    PLoS ONE, 2014-07-22;9(7):e103254.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Generation and preclinical characterization of a Fc-optimized GITR-Ig fusion protein for induction of NK cell reactivity against leukemia.
    Authors: Schmiedel, Benjamin, Werner, Antje, Steinbacher, Julia, Nuebling, Tina, Buechele, Corina, Grosse-Hovest, Ludger, Salih, Helmut R
    Mol Ther, 2013-02-05;21(4):877-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. CD26 expression and adenosine deaminase activity in regulatory T cells (Treg) and CD4(+) T effector cells in patients with head and neck squamous cell carcinoma.
    Authors: Mandapathil M, Szczepanski M, Harasymczuk M, Ren J, Cheng D, Jackson E, Gorelik E, Johnson J, Lang S, Whiteside T
    Oncoimmunology, 2012-08-01;1(5):659-669.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Human anti-inflammatory macrophages induce Foxp3+ GITR+ CD25+ regulatory T cells, which suppress via membrane-bound TGFbeta-1.
    Authors: Savage ND, De Boer T, Walburg KV, Joosten SA, van Meijgaarden K, Geluk A, Ottenhoff TH
    J. Immunol., 2008-08-01;181(3):2220-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay
  8. Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions.
    Authors: Baltz KM, Krusch M, Bringmann A, Brossart P, Mayer F, Kloss M, Baessler T, Kumbier I, Peterfi A, Kupka S, Kroeber S, Menzel D, Radsak MP, Rammensee HG, Salih HR
    FASEB J., 2007-03-14;21(10):2442-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
    Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
    Eur. J. Immunol., 2004-12-01;34(12):3485-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-09-16;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  11. Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
    Authors: Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
    J. Immunol., 2004-05-01;172(9):5213-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human GITR/TNFRSF18 Antibody
By Paul Fisher on 06/29/2016
Application: Flow Sample Tested: Transfected cells Species: Human

looked for expression of GITR on a tranfected overexpressing cell line and it worked well versus an isotype control by flow cytometry