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Human GITR/TNFRSF18 PE-conjugated Antibody

Catalog #: FAB689P
11 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB689P-100
FAB689P-025
Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GITR/TNFRSF18 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 110416
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
Gln26-Glu161 (Thr43Ala)
Accession # Q9Y5U5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
- Human peripheral blood mononuclear cells (PBMCs) treated with PHA

- RPMI8226 cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GITR/TNFRSF18 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 1 µg/mL PHA for 24 hours were stained with Mouse Anti-Human GITR/TNFRSF18 PE-conjugated Mono-clonal Antibody (Catalog # FAB689P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of GITR/TNFRSF18 in RPMI8226 (positive cells) vs. Jurkat (negative cells) by Flow Cytometry RPMI8226 cells (filled histogram) vs. Jurkat cells (open histogram) were stained with Mouse Anti-Human GITR/TNFRSF18 PE‑conjugated Monoclonal Antibody (Catalog # FAB689P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up‑regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

References
  1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  3. Gurney, A.L. et al. (1999) Current Biology 9:215.
  4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.
Long Name
Glucocorticoid Induced TNF Receptor Family Related Gene
Entrez Gene IDs
8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
Alternate Names
Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18

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Citations for Human GITR/TNFRSF18 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Human HLA-DR+CD27+ regulatory T cells show enhanced antigen-specific suppressive function
    Authors: Ma, X;Cao, L;Raneri, M;Wang, H;Cao, Q;Zhao, Y;Bediaga, NG;Naselli, G;Harrison, LC;Hawthorne, WJ;Hu, M;Yi, S;O'Connell, PJ;
    JCI insight
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CD27-Expressing Xenoantigen-Expanded Human Regulatory T Cells Are Efficient in Suppressing Xenogeneic Immune Response
    Authors: L Cao, X Ma, J Zhang, M Yang, Z He, C Yang, S Li, P Rong, W Wang
    Cell Transplantation, 2023-01-01;32(0):9636897221149.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Vitamin C supports conversion of human &gamma&delta T cells into FOXP3-expressing regulatory cells by epigenetic regulation
    Authors: L Kouakanou, C Peters, Q Sun, S Floess, J Bhat, J Huehn, D Kabelitz
    Sci Rep, 2020-04-16;10(1):6550.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Therapeutic immune monitoring of CD4(+)CD25(+) T cells in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors
    Authors: Z Lu, N Xu, X Zhou, G Gao, L Li, J Huang, Y Li, Q Lu, B He, C Pan, X Liu
    Oncol Lett, 2017-06-01;14(2):1363-1372.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Statins increase the frequency of circulating CD4+ FOXP3+ regulatory T cells in healthy individuals.
    Authors: Rodriguez-Perea A, Montoya C, Olek S, Chougnet C, Velilla P
    J Immunol Res, 2015-02-22;2015(0):762506.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  6. Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation.
    Authors: Lages CS, Suffia I, Velilla PA, Huang B, Warshaw G, Hildeman DA, Belkaid Y, Chougnet C
    J. Immunol., 2008-08-01;181(3):1835-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Antigen-induced regulatory T cells in HBV chronically infected patients.
    Authors: Barboza L, Salmen S, Goncalves L, Colmenares M, Peterson D, Montes H, Cartagirone R, Gutierrez Mdel C, Berrueta L
    Virology, 2007-07-23;368(1):41-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Effects of the administration of high-dose interleukin-2 on immunoregulatory cell subsets in patients with advanced melanoma and renal cell cancer.
    Authors: van der Vliet HJ, Koon HB, Yue SC, Uzunparmak B, Seery V, Rudensky AY, Gavin MA, Atkins MB, Balk SP, Exley MA
    Clin. Cancer Res., 2007-04-01;13(7):2100-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans.
    Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS
    J. Immunol., 2006-11-01;177(9):5811-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-09-16;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Flow Cytometry
  11. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-03-18;104(2):453-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human GITR/TNFRSF18 PE‑conjugated Antibody
By SOjan Abraham on 01/21/2016
Application: Flow Sample Tested: T regulatory cells Species: Human