Human GluR7/GRIK3 Antibody

Catalog #: MAB113281 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB113281-100
MAB113281-SP

Detection of GluR7/GRIK3 in CHO cells by Flow Cytometry.

1 Image

All GluR7/GRIK3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GluR7/GRIK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GluR7/GRIK3 in direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2914A

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human GluR7/GRIK3
Gly249-Met1
Accession # Q13003

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

CHO cells transfected with hGRIK3 vs irrelevant CHO transfectant cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

View Larger

Detection of GluR7/GRIK3 in CHO cells by Flow Cytometry. CHO cells transfected with hGRIK3 (filled histogram) vs irrelevant CHO transfectant cells (open histogram) were stained with Rabbit Anti-Human GluR7/GRIK3 Monoclonal Antibody (Catalog # MAB113281) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GluR7/GRIK3

GluR7 is a receptor for glutamate. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain. This gene can co-assemble with GRIK4 or GRIK5 to form heteromeric receptors. GluR7 is one of the five subtypes of kainate receptors. Kainate receptors are widely expressed in the CNS and are involved in the regulation of transmitter release, synapse formation, and in the pathophysiology of brain diseases. Genetic variants in the GluR7 gene are associated with schizophrenia, major depression, and bipolar disorder.

References

Entrez Gene: GRIK3 glutamate receptor, ionotropic, kainate 3.
Matute C. "Therapeutic Potential of Kainate Receptors". CNS Neuroscience & Therapeutics. 2011 Dec; 17(6):661.
Begni S, Popoli M, Moraschi S, Bignotti S, Tura G.B, Gennarelli M., "Association Between the Ionotropic Glutamate Receptor Kainate 3 (GRI3) Ser310Ala Polymorphism and Schizophrenia". Mol Psychiatry. 2002; 7(4):416.

Long Name

Glutamate Receptor 7

Entrez Gene IDs

2899 (Human); 14807 (Mouse); 298521 (Rat)

Alternate Names

EAA5; Excitatory amino acid receptor 5; GLR7; GluK3; GluR7; gluR-7; GluR7a; GLUR7dJ1090M5.1 (glutamate receptor, ionotropic, kainate 3 (GLUR7)); Glutamate receptor 7; glutamate receptor, ionotropic kainate 3; glutamate receptor, ionotropic, kainate 3; GRIK3