Human Glut1 PerCP-conjugated Antibody

Catalog #: FAB1418C Datasheet / COA / SDS

Discontinued Product

FAB1418C has been discontinued.
View all Glut1 products.
Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry.
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Human Glut1 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
Source
Monoclonal Mouse IgG2B Clone # 202915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glut1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1418C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut1

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

References
  1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
  2. Meuckler, M. et al. 1985, Science 229:941.
  3. Jones, K.S. et al. 2006, J. Virol. 8291.
  4. Takenouchi, N. et al. 2007, J. Virol. 1506.
  5. Kinet, S. et al. 2007, Retrovirology 4:31.
Long Name
Glucose Transporter Type 1
Entrez Gene IDs
6513 (Human); 20525 (Mouse)
Alternate Names
Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis); CSE; DYT17; DYT18; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; HepG2 glucose transporter; HTLVR; Human T-Cell Leukemia Virus (I and II) Receptor; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; Solute Carrier Family 2 Member 1; solute carrier family 2, facilitated glucose transporter member 1

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