Human Glut4 Antibody

Catalog #: MAB86541 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB86541-100
MAB86541-SP

Detection of Glut4 in HEK293 Human Cell Line Transfected with Human Glut4 and eGFP by Flow Cytometry.

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All Glut4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glut4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 925932

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glut4
Met1-Asp509
Accession # P14672

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Glut4 in HEK293 Human Cell Line Transfected with Human Glut4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Glut4 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glut4 Monoclonal Antibody (Catalog # MAB86541) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut4

Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a 509 aa multi-pass type membrane protein and shares 65% aa identity with mouse Glut4. It is an insulin-regulated glucose transporter. Glut4 is most highly expressed in adipose and striated muscle tissues, but has also been reported in multiple other tissues including the nervous system and breast cancer. Insulin stimulated transport of Glut4 has been shown to be impaired in type 2 diabetes patients. Additionally, inhibition of Glut4 may be useful in reducing proliferation of breast cancer cells.

Entrez Gene IDs

6517 (Human); 20528 (Mouse); 25139 (Rat)

Alternate Names

Glut4; GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4