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Human Glut5 Antibody

Catalog #: MAB1349
3 Citations Datasheet / COA / SDS
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MAB1349
MAB1349-SP
Detection of Glut5 in NS0 Mouse Cell Line Transfected with Human Glut5 by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut5. Stains human Glut5 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 195205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut5
Met1-Gln501
Accession # P22732
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human small intestine and skeletal muscle
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glut5 antibody in NS0 Mouse Cell Line Transfected with Human Glut5 antibody by Flow Cytometry. View Larger

Detection of Glut5 in NS0 Mouse Cell Line Transfected with Human Glut5 by Flow Cytometry. NS0 mouse myeloma cell line either transfected with human Glut5 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human Glut5 Monoclonal Antibody, followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut5

Glut5 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues. Glut5 is expressed at highest levels in small intestine and at lower levels in kidney, testis, skeletal muscle, and adipose tissue (1, 2). Glut5 transports fructose in intestine, testis, and other tissues.

References
  1. Kayano, T. et al. (1990) J. Biol. Chem. 265:13276.
  2. Corpe, C.P. (2002) Biochem. Biophys. Acta. 1576:191.
  3. Sasaki, A. et al. (2003) Neurosci. Lett. 338:17.
Long Name
Glucose Transporter Type 5
Entrez Gene IDs
6518 (Human); 56485 (Mouse)
Alternate Names
Fructose transporter; Glucose transporter type 5, small intestine; Glut5; GLUT-5; GLUT5glucose transporter-like protein 5; SLC2A5; solute carrier family 2 (facilitated glucose transporter), member 5; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; solute carrier family 2, facilitated glucose transporter member 5

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Citations for Human Glut5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. PIP2 depletion and altered endocytosis caused by expression of Alzheimer's disease‐protective variant PLC gamma 2 R522
    Authors: Emily Maguire, Georgina E Menzies, Thomas Phillips, Michael Sasner, Harriet M Williams, Magdalena A Czubala et al.
    The EMBO Journal
  2. Diabetes mellitus and prostate cancer metabolism: Is there a relationship?
    Authors: Hugo Pontes Antunes, Ricardo Teixo, João André Carvalho, Miguel Eliseu, Inês Marques, Ana Mamede et al.
    Archivio Italiano di Urologia e Andrologia
  3. Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression
    Authors: Ana F. Brito, Ana M. Abrantes, Marina Ribeiro, Rui Oliveira, João Casalta-Lopes, Ana C. Gonçalves et al.
    Journal of Clinical and Experimental Hepatology

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