Home / Glycophorin A / Human Glycophorin A PE-conjugated Antibody

Human Glycophorin A PE-conjugated Antibody

Catalog #: FAB12281P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB12281P
Detection of Glycophorin A in TF-1 Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glycophorin A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].
Source
Monoclonal Mouse IgG1 Clone # R10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced human pre-B tumor cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glycophorin A antibody in TF-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glycophorin A in TF-1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Glycophorin A PE-conjugated Monoclonal Antibody (Catalog # FAB12281P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glycophorin A

Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).

References
  1. Greaves, M.F. et al. (1983) Blood 61(4):645.
Entrez Gene IDs
2993 (Human)
Alternate Names
CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR

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Citations for Human Glycophorin A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
    Authors: A Borojevi?, A Jaukovi?, T Kukolj, S Mojsilovi?, H Obradovi?, D Trivanovi?, M Živanovi?, Ž Ze?evi?, M Simi?, B Gobelji?, D Vuji?, D Bugarski
    Biomolecules, 2022-02-18;12(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Targeted erythropoietin selectively stimulates red blood cell expansion in vivo.
    Authors: DR Burrill, Burrill D, A Vernet, Vernet A, JJ Collins, PA Silver, JC Way, Way J
    Proc Natl Acad Sci U S A, 2016-04-25;113(19):5245-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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