Human Glycoprotein V/CD42d PE-conjugated Antibody Summary
Gln17-Gly523
Accession # P40197
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Glycoprotein V/CD42d in Human Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Anti-Human CD41 FITC-conjugated Antibody and either (A) Mouse Anti-Human Glycoprotein V/CD42d PE-conjugated Monoclonal Antibody (Catalog # FAB4249P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glycoprotein V/CD42d
GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine‑rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI‑V‑IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard‑Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).
- Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
- Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
- Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
- Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
- Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
- Wolff, V. et al. (2005) Stroke 36:e17.
- Javela, K. et al. (2005) Transfusion 45:1504.
- Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
- Kahn, M.L. (1999) Blood 94:4112.
- Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
- Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
- Moog, S. et al. (2001) Blood 98:1038.
- Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
- Ni, H. et al. (2001) Blood 98:368.
- Andrews, R.K. et al. (2001) Blood 98:681.
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