Human Glypican 6 Antibody

Catalog #: MAB2845
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2845
MAB2845-SP

Detection of Glypican 6 in HepG2 Human Cell Line by Flow Cytometry.

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All Glypican 6 Antibodies

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Human Glypican 6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glypican 6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Glypican 2, 3, or 5 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 348701

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glypican 6
Asp24-Val527
Accession # Q9Y625

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Glypican 6 (Catalog # 2845-GP)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Glypican 6 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

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Detection of Glypican 6 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glypican 6 Monoclonal Antibody (Catalog # MAB2845, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 6

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1-4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C‑terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is down‑regulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9).

References

Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
Filmus, J. (2001) Glycobiology 11:19R.
De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
Filmus, J. (2003) Glycoconj. J. 19:319.
Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
Paine-Saunders, S. et al. (1999) Genomics 57:455.
Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
Bassett, J.H.D. et al. (2006) Endocrinology 147:295.

Entrez Gene IDs

10082 (Human); 23888 (Mouse)

Alternate Names

Glypican 6; glypican proteoglycan 6; glypican-6; GPC6; MGC126288; OMIMD1

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Citation for Human Glypican 6 Antibody

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