Home / Goosecoid / Human Goosecoid Antibody

Human Goosecoid Antibody

Catalog #: AF4086
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4086
AF4086-SP
Goosecoid in BG01V Human Embryonic Stem Cells.
1 Image
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Goosecoid Antibody Summary

Species Reactivity
Human
Specificity
Detects human Goosecoid in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Goosecoid
Gly48-Arg159
Accession # P56915
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Goosecoid
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Goosecoid antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Goosecoid in BG01V Human Embryonic Stem Cells. Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Goosecoid

Goosecoid (GSC) is a 28 kDa member of the paired homeobox family. Human Goosecoid is 257 amino acids (aa) in length, and contains one DNA-binding homeodomain (aa 160‑219). Goosecoid appearance correlates with the start of gastrulation and the development of both head/neck and upper body limb mesenchyme. It also appears abnormally in some tumors where it is associated with an epithelial-to-mesenchymal transition. Human Goosecoid shares 97% aa sequence identity with mouse Goosecoid.

Entrez Gene IDs
145258 (Human); 14836 (Mouse); 317715 (Rat)
Alternate Names
goosecoid homeobox; Goosecoid; GSC; homeobox protein goosecoid

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Goosecoid Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

  1. Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway
    Authors: Jaroslav Fabik, Katarina Kovacova, Zbynek Kozmik, Ondrej Machon
    Biology Open
  2. Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip
    Authors: Musah S, Mammoto A, Ferrante TC et al.
    Nat Biomed Eng.
  3. Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Authors: Bhattacharya, R;Ward, T;Kalejaiye, TD;Mishra, A;Leeman, S;Arzaghi, H;Seidman, JG;Seidman, CE;Musah, S;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Transfected Whole Cells
    Applications: Western Blot, Immunocytochemistry
  4. ARID1A deficiency weakens BRG1-RAD21 interaction that jeopardizes chromatin compactness and drives liver cancer cell metastasis
    Authors: XY Shang, Y Shi, DD He, L Wang, Q Luo, CH Deng, YL Qu, N Wang, ZG Han
    Cell Death & Disease, 2021-10-23;12(11):990.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  5. WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids
    Authors: A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou
    Elife, 2018-10-12;7(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip
    Authors: S Musah, N Dimitrakak, DM Camacho, GM Church, DE Ingber
    Nat Protoc, 2018-07-01;13(7):1662-1685.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System
    Authors: K Low, LY Wong, M Maldonado, C Manjunath, CB Horner, M Perez, NV Myung, J Nam
    Stem Cell Reports, 2017-04-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Induced hepatic stem cells are suitable for human hepatocyte production
    Authors: Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al.
    iScience

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Goosecoid Antibody

There are currently no reviews for this product. Be the first to review Human Goosecoid Antibody and earn rewards!

Have you used Human Goosecoid Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review