Human gp130 PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of gp130 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human gp130 PE-conjugated Monoclonal Antibody (Catalog # FAB228P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: gp130
Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).
2. Muller-Newen, G. (2003) Sci. STKE pe40.
3. Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
Product Datasheets
Citations for Human gp130 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Interleukin 6/gp130 axis promotes neural invasion in pancreatic cancer
Authors: H Suzuki, S Mitsunaga, M Ikeda, T Aoyama, K Yoshizawa, M Yamaguchi, M Suzuki, M Narita, T Kawasaki, A Ochiai
Cancer Medicine, 2022-05-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Plasminogen Activator Inhibitor-1 Promotes the Recruitment and Polarization of Macrophages in Cancer
Authors: MH Kubala, V Punj, VR Placencio-, H Fang, GE Fernandez, R Sposto, YA DeClerck
Cell Rep, 2018-11-20;25(8):2177-2191.e7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The IL-6 trans-signaling-STAT3 pathway mediates ECM and cellular proliferation in fibroblasts from hypertrophic scar.
Authors: Ray S, Ju X, Sun H, Finnerty C, Herndon D, Brasier A
J Invest Dermatol, 2013-01-10;133(5):1212-20.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
Authors: Cocco C, Di Carlo E, Zupo S
Leukemia, 2011-12-23;26(6):1365-74.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
Mol. Cell. Biol., 2010-06-28;17(0):4175.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-6 regulates MCP-1, ICAM-1 and IL-6 expression in human myoblasts.
Authors: Marino M, Scuderi F, Provenzano C, Scheller J, Rose-John S, Bartoccioni E
J. Neuroimmunol., 2008-04-08;196(1):41-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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