Human GPR115 Antibody

Catalog #: MAB5437 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5437
MAB5437-SP

Detection of Human GPR115 by Western Blot.

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All GPR115 Antibodies

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Human GPR115 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR115 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human GPR30, 49, 56, 111, 114, 124, or 125 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 527003

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human GPR115
Ser22-Ala347
Accession # Q8IZF3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GPR115 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human GPR115 Monoclonal Antibody (Catalog # MAB5437) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GPR115 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR115

GPR115 is a member of the LN-7TM family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a long extracellular N-terminus (1, 2). The 695 amino acid (aa) human GPR115 sequence predicts a 21 aa signal sequence, a 385 aa N‑terminal extracellular domain (ECD), seven TM regions separated by 6‑24 aa intracellular and extracellular regions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECD contains a highly glycosylated mucin‑like stalk that is predicted to function in adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavage site (GPS) (1). GPS domains, which have been described in other 7TM proteins including ETL, GPR126, HE6, and Latrophilin-1, are cleavage sites for processing proteins into two subunits (3‑7). Within the N‑terminal region that ends with the predicted cleavage site (aa 22‑347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract (1).

References

Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
Nechiporuk, T. et al. (2001) J. Biol. Chem. 276:4150.
Moriguchi, T. et al. (2004) Genes Cells 9:549.
Kierszenbaum, A.L. (2003) Mol. Reprod. Dev. 64:1.
Krasnoperov, V.G. et al. (1997) Neuron 18:925.
Krasnoperov, V. et al. (2002) J. Biol. Chem. 277:46518.

Long Name

G Protein-coupled Receptor 115

Entrez Gene IDs

221393 (Human); 78249 (Mouse); 501106 (Rat)

Alternate Names

FLJ38076; G protein-coupled receptor 115; GPR115; G-protein coupled receptor PGR18; PGR18; PGR18G-protein coupled receptor 115; probable G-protein coupled receptor 115; seven transmembrane helix receptor