Human GPR158 Antibody

Name: Human GPR158 Antibody
Brand: R&D Systems
SKU: MAB10286
Rating: 5 (1 reviews)

Catalog #: MAB10286 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10286-100
MAB10286-SP

Detection of Human GPR158 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR158 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR158 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1027604

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human GPR158 protein
Ala24-Gln411
Accession # Q5T848

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

HEK293T human embryonic kidney cell line transfected with human GPR158

Simple Western

25 µg/mL

HEK293T human embryonic kidney cell line transfected with GPR-158 and eGFP

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human GPR158 and eGFP

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cortex) tissue

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GPR158 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human GPR158. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GPR158 Monoclonal Antibody (Catalog # MAB10286) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR158 at approximately 175 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry

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Detection of GPR158 in HEK293 Human Cell Line Transfected with Human GPR158 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPR158 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR158 Monoclonal Antibody (Catalog # MAB10286) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunohistochemistry

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GPR158 in Human Brain Cortex Tissue. GPR158 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) tissue using Mouse Anti-Human GPR158 Monoclonal Antibody (Catalog # MAB10286) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of GPR158 by Simple Western^TM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with GPR-158 and eGFP, loaded at 0.2 mg/mL. A specific band was detected for GPR158 at approximately 191 kDa (as indicated) using 25 µg/mL of Mouse Anti-Human GPR158 Monoclonal Antibody (Catalog # MAB10286). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR158

G-protein coupled receptor 158 (GPR158) is a receptor belonging to the Class C GPCR family. It lacks the extracellular Venus flytrap module characteristic of the known members of that family and instead contains two other elements that are not typical of the class: a calcium-binding EGF-like domain and a leucine repeat region (1, 2). The mature extracellular domain of human GPR158 contains 393 amino acids (aa) and shares 89% identity with both mouse and rat GPR158. GPR158 is expressed at the highest level in the brain, but also in a variety of other tissues including retina, spleen, liver and lung (3). GPR158 was originally identified in functional screens linked with biological stress and has been implicated in the osteocalcin effect on cognitive processes in the brain (4, 5), and glaucoma and cancer in the periphery (4, 6).

References