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Human GPR35 Antibody

Catalog #: MAB10181 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10181-100
MAB10181-SP
Detection of Human GPR35 by Western Blot.
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Human GPR35 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR35 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1007216
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human GPR35
Accession # Q9HC97
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GPR35 antibody by Western Blot. View Larger

Detection of Human GPR35 by Western Blot. Western blot shows lysates of human ileum tissue and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR35 Monoclonal Antibody (Catalog # MAB10181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GPR35 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR35

Human G protein-coupled receptor 35 also known as GPR35 (also known as Kynurenic acid or KYNA receptor) is a 7-transmembrane domain G protein-coupled receptor encoded by the GPR35 gene. Polymorphisms in the G protein–coupled receptor GPR35 are associated with increased risk for certain inflammatory diseases of the bile duct and large intestine that have increased cancer risk.

Long Name
G Protein-coupled Receptor 35
Entrez Gene IDs
2859 (Human); 64095 (Mouse); 367315 (Rat)
Alternate Names
G protein-coupled receptor 35; GPR35; G-protein coupled receptor 35; KYNA receptor; Kynurenic acid receptor

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