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Human GPR50 Antibody

Catalog #: MAB4645 Datasheet / COA / SDS

Discontinued Product

MAB4645 has been discontinued.
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GPR50 in A172 Human Cell Line.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR50 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR50. Stains human GPR50 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 461129
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR50
Met1-Val617
Accession # Q13585
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry GPR50 antibody in A172 Human Cell Line by Immunocytochemistry (ICC). View Larger

GPR50 in A172 Human Cell Line. GPR50 was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Mouse Anti-Human GPR50 Monoclonal Antibody (Catalog # MAB4645) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of GPR50 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of GPR50 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR50 Monoclonal Antibody (Catalog # MAB4645, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR50

GPR50, also known as MTR1L, is a non-glycosylated seven-transmembrane G protein-coupled receptor that is related to the melatonin receptors MT1 and MT2. GPR50 is expressed in the hippocampus, hypothalamus, and pituitary and forms 130 kDa homodimers. It heterodimerizes with either MT1 or MT2, resulting in inhibition of MT1 but not MT2 function. An alternately spliced isoform of GPR50 has a 4 aa deletion in the large C-terminal cytoplasmic domain. The presence of this deletion as well as various polymorphisms have been associated with elevated serum triglyceride and HDL levels. The deletion may also be associated with the development of bipolar disorder. Human GPR50 shares approximately 70% amino acid sequence identity with mouse and rat GPR50.

Long Name
G Protein-coupled Receptor 50
Entrez Gene IDs
9248 (Human); 14765 (Mouse); 117097 (Rat)
Alternate Names
G protein-coupled receptor 50H9Mel1c; GPR50; H9; melatonin-related receptor; MGC125342; MTR1L

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