Human GPR52 Antibody

Catalog #: MAB10357 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10357-100
MAB10357-SP

Detection of GPR52 in HEK293 Human Cell Line Transfected with Human GPR52 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR52 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR52 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 582671

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR52
Met1-Ile361
Accession # Q9Y2T5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPR52 in HEK293 Human Cell Line Transfected with Human GPR52 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GPR52 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR52 Monoclonal Antibody (Catalog # MAB10357) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on isotype control antibody (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR52

GPR52 is a class-A orphan G-protein-coupled receptor (GPCR) that is highly expressed in the brain and represents a promising therapeutic target for the treatment of several neuropsychiatric disorders, including Huntington's disease and schizophrenia. GPR52 is a 41KDa seven-transmembrane domain protein (7TM). Mouse and human sequences are 96% identical over the full protein.

Long Name

G-Protein-Coupled Receptor 52

Entrez Gene IDs

9293 (Human); 620246 (Mouse); 680192 (Rat)

Alternate Names

G protein-coupled receptor 52; GPR52; MGC111751; probable G-protein coupled receptor 52