Human GPR75 Antibody

Catalog #: MAB67281 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB67281-100
MAB67281-SP

Detection of GPR75 in HEK293 Human Cell Line Transfected with Human GPR75 and eGFP by Flow Cytometry.

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Human GPR75 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR75 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 689714

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR75
Accession # O95800

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human GPR75 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPR75 in HEK293 Human Cell Line Transfected with Human GPR75 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPR75 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR75 Monoclonal Antibody (Catalog # MAB67281) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR75

GPR75 is a 540 amino acid, 7-transmembrane G-protein-coupled glycoprotein receptor mainly expressed in retinal pigment epithelial cells and brain. Polymorphisms in humans have been associated with macular degeneration. GPR75 has been identified as a receptor for CCL5/RANTES in the brain. Collectively, the extracellular domains of human GPR75 share 75% amino acid sequence identity with the corresponding regions of mouse and rat GPR75.

Long Name

G Protein-coupled Receptor 75

Entrez Gene IDs

10936 (Human); 237716 (Mouse); 498434 (Rat)

Alternate Names

G protein-coupled receptor 75; GPR75; GPRchr2; MGC132002; probable G-protein coupled receptor 75; WI31133; WI-31133