Human GPR85 Antibody

Catalog #: MAB10761 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10761-100
MAB10761-SP

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Human GPR85 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR85 in cell based ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1042202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GPR85 extracellular domain synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GPR85 in U‑87 MG Human Cell Line. GPR85 was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Mouse Anti-Human GPR85 Monoclonal Antibody (Catalog # MAB10761) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR85

Long Name

G Protein-coupled Receptor 85

Entrez Gene IDs

54329 (Human); 64450 (Mouse); 64020 (Rat)

Alternate Names

FLJ50993; FLJ51159; FLJ99433; G protein-coupled receptor 85; GPR85; probable G-protein coupled receptor 85; seven transmembrane helix receptor; SREB; SREB2; SREB2Super conserved receptor expressed in brain 2