Human GPR88 Antibody

Catalog #: MAB10828 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10828-100
MAB10828-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR88 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR88 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1039830

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GPR88 synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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GPR88 in Human Brain (Caudate Nucleus). GPR88 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human GPR88 Monoclonal Antibody (Catalog # MAB10828) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR88

GPR88 is an orphan receptor that probably belongs to the G-protein coupled receptor family. Ligands for this receptor still largely remain unknown but agonists have been discovered. It is a brain-specific receptor with almost exclusive expression in the striatum, being preferentially localized in medium spiny neurons. Because of its expression, GPR88 is emerging as a drug target for CNS disorders such as schizophrenia, Parkinson's disease, anxiety, and addiction. Gpr88 KO mice data suggests that striatal GPR88 receptors influence activity in a range of procedures integrated by prefrontal, orbitofrontal and anterior cingulate cortico-striatal-thalamic loops leading to altered cognitive, motivational and reward evaluation processes.

Long Name

G Protein-coupled Receptor 88

Entrez Gene IDs

54112 (Human); 64378 (Mouse); 64443 (Rat)

Alternate Names

G protein coupled receptor 88; G protein-coupled receptor 88; GPR88; G-protein coupled receptor 88; probable G-protein coupled receptor 88; STRG; Striatum-specific G-protein coupled receptor