Human GPRC5D PE-conjugated Antibody

Name: Human GPRC5D PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB6300P

Catalog #: FAB6300P Datasheet / COA / SDS

Newer Version Available: FAB6300RP

NEW

Discontinued Product

FAB6300P has been discontinued and is replaced by FAB6300RP.

Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry.

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Human GPRC5D PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPRC5D in direct ELISAs. Stains human GPRC5D transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 571961

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPRC5D
Met1-Val359
Accession # Q9NZD1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPRC5D or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPRC5D PE-conjugated Monoclonal Antibody (Catalog # FAB6300P) or Mouse IgG_2BPE-conjugated Isotype Control (Catalog # IC0041P, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPRC5D

G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.

Long Name

G Protein-coupled Receptor, Family D, Group 5, Member D

Entrez Gene IDs

55507 (Human); 93746 (Mouse); 500349 (Rat)

Alternate Names

G protein-coupled receptor, family C, group 5, member D; GPRC5D; G-protein coupled receptor family C group 5 member D; MGC129713; MGC129714; orphan G-protein coupled receptor