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Human GRAIL/RNF128 Antibody

Catalog #: AF6234
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6234
AF6234-SP
Detection of Human GRAIL/RNF128 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GRAIL/RNF128 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GRAIL/RNF128 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GRAIL/RNF128
Val42-Pro161
Accession # Q8TEB7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GRAIL/RNF128 antibody by Western Blot. View Larger

Detection of Human GRAIL/RNF128 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human GRAIL/RNF128 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRAIL/RNF128 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRAIL/RNF128

GRAIL (Gene related to anergy in lymphocytes protein; also RNF128) is a 45-47 kDa, type I transmembrane E3 ubiquitin ligase. It is expressed in the endosomes of select cell types such as early myeloid progenitors and lymphocytes, and mediates the ubiquitination of membrane proteins such as CD3, CD40L, and CD83, plus the tetraspanins CD81 and CD151. GRAIL apparently regulates the threshold for T cell activation, and as such, plays a key role in autoimmunity. In Treg cells, GRAIL promotes suppressive activity by restricting Th17 gene expression. Mature human GRAIL is 390 amino acids (aa) in length (aa 39-428). It possesses a 169 aa luminal region (aa 39-207) and a 190 aa cytoplasmic domain. The luminal region contains one PA (protease-associated) domain that mediates protein-protein interactions (aa 48-193), and the cytoplasmic segment shows one zinc-finger region (aa 277-318). There is one splice variant that shows a 134 aa substitution for aa 1-161. Over aa 42-161, human GRAIL shares 98% aa identity with mouse GRAIL.

Long Name
Gene Related to Anergy in Lymphocytes Protein/Ring Finger Protein 128
Entrez Gene IDs
79589 (Human); 66889 (Mouse); 315911 (Rat)
Alternate Names
EC 6.3.2; EC 6.3.2.-; FLJ23516; Gene related to anergy in lymphocytes protein; GRAIL; ring finger protein 128GRAILE3 ubiquitin-protein ligase RNF128; RNF128

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Citation for Human GRAIL/RNF128 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Isoforms of RNF128 Regulate the Stability of Mutant P53 in Barrett's Esophageal Cells
    Authors: Dipankar Ray, Paramita Ray, Daysha Ferrer-Torres, Zhuwen Wang, Derek Nancarrow, Hee-won Yoon et al.
    Gastroenterology

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