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Human Granzyme A PE-conjugated Antibody

Catalog #: IC29051P
1 Citation Datasheet / COA / SDS

Discontinued Product

IC29051P has been discontinued.
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Detection of Granzyme A in CD56+Human Blood Cells by Flow Cytometry.
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Human Granzyme A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Granzyme A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Granzyme B, C, D, G, or H is observed.
Source
Monoclonal Mouse IgG2B Clone # 356412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme A
Cys26-Val262
Accession # P12544
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Granzyme A antibody in CD56<SUP>+</SUP>Human Blood Cells antibody by Flow Cytometry. View Larger

Detection of Granzyme A in CD56+Human Blood Cells by Flow Cytometry. CD56+human whole blood cells were stained with Mouse Anti-Human Granzyme A PE-conjugated Monoclonal Antibody (Catalog # IC29051P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Granzyme A

Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1‑26), a propeptide (residues 27‑28) and a mature chain (residues 29‑262) (2). The purified recombinant human Granzyme A consists of residues 26 to 262. After being activated by lysyl endopeptidase, it cleaves a thioester substrate.

References
  1. Lieberman, J. and Z. Fan (2003) Curr. Opin. Immunol. 15:553.
  2. Gershenfeld, H.K. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1184.
Entrez Gene IDs
3001 (Human); 14938 (Mouse); 266708 (Rat)
Alternate Names
CTL Tryptase; CTLA3; CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease); Cytotoxic T-lymphocyte proteinase 1; EC 3.4.21; Fragmentin-1; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme A; Granzyme-1; GZMA; H factor; Hanukkah factor; HF; HFSP; HFSPEC 3.4.21.78

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Citation for Human Granzyme A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The IL-7R alpha pathway is quantitatively and functionally altered in CD8 T cells in multiple sclerosis.
    Authors: Kreft KL, Verbraak E, Wierenga-Wolf AF, van Meurs M, Oostra BA, Laman JD, Hintzen RQ
    J. Immunol., 2012-01-18;188(4):1874-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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