Home / Granzyme B / Human Granzyme B PE-conjugated Antibody

Human Granzyme B PE-conjugated Antibody

Catalog #: IC2906P
5 Citations Datasheet / COA / SDS
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IC2906P
Detection of Granzyme B in NK‑92 Human Cell Line by Flow Cytometry.
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Citations (5)
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Human Granzyme B PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Granzyme B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.
Source
Monoclonal Mouse IgG2A Clone # 351927
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Granzyme B antibody in NK‑92 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Granzyme B in NK‑92 Human Cell Line by Flow Cytometry. NK-92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B PE-conjugated Monoclonal Antibody (Catalog # IC2906P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Granzyme B

Granzyme B is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of Cytotoxic T Lymphocytes (CTL) and Natural Killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various Caspases and cleaves proteins such as Aggrecan (3). Human Granzyme B is synthesized as a 247 amino acid (aa) precursor with a signal peptide (aa 1-18), a pro peptide (aa 19-20), and a mature chain (aa 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of aa 19-247 was expressed and purified. After being activated by active Cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).

References
  1. Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549 - 1552.
  4. Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
  5. Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
  6. Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.
Entrez Gene IDs
3002 (Human); 14939 (Mouse)
Alternate Names
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT

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Citations for Human Granzyme B PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Transcriptomic analysis of human IL-7 receptor alpha low and high effector memory CD8+ T cells reveals an age-associated signature linked to influenza vaccine response in older adults
    Authors: HJ Park, MS Shin, M Kim, JB Bilsborrow, S Mohanty, RR Montgomery, AC Shaw, S You, I Kang
    Aging Cell, 2019-05-01;0(0):e12960.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Anti-CD137 monoclonal antibody enhances trastuzumab-induced, natural killer cell-mediated cytotoxicity against pancreatic cancer cell lines with low human epidermal growth factor-like receptor 2 expression
    Authors: T Masu, M Atsukawa, K Nakatsuka, M Shimizu, D Miura, T Arai, H Harimoto, C Kondo, K Kaneko, S Futagami, C Kawamoto, H Takahashi, K Iwakiri
    PLoS ONE, 2018-12-31;13(12):e0200664.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Humoral and cell-mediated immune responses after a booster dose of HBV vaccine in HIV-infected children, adolescents and young adults
    Authors: V Giacomet, M Masetti, P Nannini, F Forlanini, M Clerici, GV Zuccotti, D Trabattoni
    PLoS ONE, 2018-02-14;13(2):e0192638.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
    Authors: Dorneles E, Lima G, Teixeira-Carvalho A, Araujo M, Martins-Filho O, Sriranganathan N, Al Qublan H, Heinemann M, Lage A
    PLoS ONE, 2015-09-09;10(9):e0136696.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.
    Authors: Peguillet I, Milder M, Louis D, Vincent-Salomon A, Dorval T, Piperno-Neumann S, Scholl S, Lantz O
    Cancer Res, 2014-02-17;74(8):2204-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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