Human Growth Hormone R/GHR Antibody

Catalog #: MAB1210
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1210
MAB1210-SP

Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Human Growth Hormone R/GHR Antibody.

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Human Growth Hormone R/GHR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Growth Hormone R/GHR in Western blots and direct ELISAs. In Western blots, no cross-reactivity with recombinant mouse GHR or recombinant rat GHR is observed.

Source

Monoclonal Mouse IgG_2B Clone # 193804

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Growth Hormone R/GHR
Phe19-Tyr264
Accession # P10912

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Growth Hormone R/GHR Fc Chimera (Catalog # 1210-GR)

Neutralization

Measured by its ability to neutralize Growth Hormone R/GHR-mediated inhibition of proliferation in the Nb2‑11 rat lymphoma cell line. The Neutralization Dose (ND₅₀) is typically 0.025-0.1 µg/mL in the presence of 10 ng/mL Recombinant Human Growth Hormone R/GHR Fc Chimera and 0.2 ng/mL Recombinant Human Growth Hormone.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Human Growth Hormone R/GHR Antibody. Recombinant Human Growth Hormone R/ GHR Fc Chimera (Catalog # 1210-GR) inhibits Recom-binant Human Growth Hormone (Catalog # 1067-GH) induced pro-liferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human Growth Hormone (0.2 ng/mL) activity elicited by Recom-binant Human Growth Hormone R/ GHR Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Growth Hormone R/GHR Monoclonal Antibody (Catalog # MAB1210). The ND₅₀ is typically 0.025-0.1 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Human GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with an 18 aa putative signal peptide, a 246 aa extracellular domain, a 24 aa transmembrane domain and a 350 aa cytoplasmic domain (5). At least two alternatively spliced isoforms of human GHR, lacking the sequence encoded by exon 3, or lacking most of the cytoplasmic domain, also exist (6, 7). Soluble GH-binding proteins corresponding to extracellular domain of the transmembrane proteins can be generated from the membrane proteins (8). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (9). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (8, 10).

References

Goffin, V. et al. (1996) Endocrine Rev. 17:385.
Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
Clark, R. (1997) Endocr. Rev. 18:157.
Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
Leung, D.W. et al. (1987) Nature 330:537.
Stallings-Mann, J.L. et al. (1996) Proc. Nat. Acad. Sci. 93:12394.
Amit, T. et al. (1997) Endocr. Metab. 82:3813.
Ross, R.J.M., et al. (1997) Molecular Endocrinology 11:265.
Carter-Su, C. et al. (1996) Annu. Rev. Physiol. 58:187.
Postel-Vinay, M.C. and J. Finidori (1995) Eur. J. Endocrinol. 133:654.

Long Name

Growth Hormone Receptor

Entrez Gene IDs

2690 (Human); 14600 (Mouse); 25235 (Rat)

Alternate Names

GH receptor; GHBP; GHR; growth hormone binding protein; Growth Hormone R; growth hormone receptor; serum binding protein; Somatotropin receptor

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Citation for Human Growth Hormone R/GHR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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