Human Guanylyl Cyclase C/GUCY2C PE-conjugated Antibody

Catalog #: FAB2157P Datasheet / COA / SDS

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FAB2157P-100

Detection of Guanylyl Cyclase C/GUCY2C in hGUCY2C eGFP/HEK293 tfx cells by Flow Cytometry.

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Human Guanylyl Cyclase C/GUCY2C PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Guanylyl Cyclase C/GUCY2C in direct ELISAs.

Source

Monoclonal Rabbit IgG Clone # 2543C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human Guanylyl Cyclase C/GUCY2C
Ser21-Gln430
Accession # P25092

Formulation

Supplied 0.005 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Guanylyl Cyclase C/GUCY2C in hGUCY2C eGFP/HEK293 tfx cells by Flow Cytometry. HEK293 transfected with Human GUCY2C and eGFP were stained with eGFP and either (A) Rabbit Anti-Human Guanylyl Cyclase C/GUCY2C PE‑conjugated Monoclonal Antibody (Catalog # FAB2157P) or (B) Rabbit IgG PE-conjugated Antibody (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

References

Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
Lucas K. et al. (2000) Pharmacol Rev. 52:375.
Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
Gong, R. et al. (2011) Science. 333:1642.
Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Alternate Names

DIAR6; EC 4.6.1; GCC; GC-C; guanylate cyclase 2C (heat stable enterotoxin receptor); Guanylate Cyclase C; Guanylyl Cyclase C; GUC2C; GUC2CEC 4.6.1.2; GUCY2C; heat-stable enterotoxin receptor; hSTAR; Intestinal guanylate cyclase; MUCIL; STA Receptor; StAR; STARSTA receptor