Human HAI-1 Antibody Summary
HAI‑1 is observed and less than 1% cross-reactivity with recombinant human HAI-2 is observed.
Pro37-Glu449
Accession # NP_003701
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human HAI‑1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HAI-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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HAI‑1 in Human Lung Cancer Tissue. HAI-1 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human HAI‑1 by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for HAI-1 at approximately 88 kDa (as indicated) using 10 µg/mL of Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAI-1
HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).
- Denda, K. et al. (2002) J. Biol. Chem. 277:14053.
- Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
- Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.
Product Datasheets
Citations for Human HAI-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Matriptase-dependent epidermal pre-neoplasm in zebrafish embryos caused by a combination of hypotonic stress and epithelial polarity defects
Authors: Hatzold, J;Nett, V;Brantsch, S;Zhang, JL;Armistead, J;Wessendorf, H;Stephens, R;Humbert, PO;Iden, S;Hammerschmidt, M;
PLoS genetics
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins
Authors: CJ Wu, M Lu, X Feng, G Nakato, MC Udey
Cells, 2020-04-21;9(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma
Authors: X Du, L Wu, MS Ur Rahman, X Teng, L Teng, J Ye, J Cao
Dis. Markers, 2019-08-28;2019(0):9175215.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Matrix metalloproteinase-7 induces homotypic tumor cell aggregation via proteolytic cleavage of the membrane-bound Kunitz-type inhibitor HAI-1
Authors: T Ishikawa, Y Kimura, H Hirano, S Higashi
J. Biol. Chem., 2017-10-18;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization
Authors: AS Murray, FA Varela, TE Hyland, AJ Schoenbeck, JM White, LM Tanabe, SV Todi, K List
J. Biol. Chem., 2017-07-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting the membrane-anchored serine protease testisin with a novel engineered anthrax toxin prodrug to kill tumor cells and reduce tumor burden
Authors: Erik W. Martin, Marguerite S. Buzza, Kathryn H. Driesbaugh, Shihui Liu, Yolanda M. Fortenberry, Stephen H. Leppla et al.
Oncotarget
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Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway.
Authors: Buzza M, Martin E, Driesbaugh K, Desilets A, Leduc R, Antalis T
J Biol Chem, 2013-02-26;288(15):10328-37.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development.
Authors: Szabo R, Molinolo A, List K, Bugge TH
Oncogene, 2006-09-18;26(11):1546-56.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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