Human HAI-1 Biotinylated Antibody

Catalog #: BAM10482 Datasheet / COA / SDS

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BAM10482

All HAI-1 Antibodies

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Human HAI-1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HAI-1 In ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse HAI-1, recombinant human (rh) TFPI, rhTFPI-2, rhAPP, rhHAI-2A, rhGASP-1, or rhGASP-2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 169428

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Val449
Accession # O43278

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human HAI-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human HAI‑1 Antibody (Catalog # MAB10481)

Standard: Recombinant Human HAI-1 Protein, CF (Catalog # 1048-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-1

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

References

Denda, K. et al. (2002) J. Biol. Chem. 277:14053.
Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.

Long Name

HGF Activator Inhibitor Type 1

Entrez Gene IDs

6692 (Human); 20732 (Mouse)

Alternate Names

HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1