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Human HAPLN4 Antibody

Catalog #: AF4085
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4085
AF4085-SP
HAPLN4 in Human Brain.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HAPLN4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HAPLN4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HAPLN1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAPLN4
Gln30-Val402
Accession # Q86UW8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HAPLN4
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human HAPLN4, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HAPLN4 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

HAPLN4 in Human Brain. HAPLN4 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using 3 µg/mL Goat Anti-Human HAPLN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4085) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAPLN4

HAPLN4 (hyaluronan and proteoglycan link protein 4; also Bral2) is a 40-42 kDa secreted member of the Link module superfamily. It is secreted by select neurons, particularly of the cerebellum, where it participates in ECM stability. Here it may link hyaluronic acid with resident proteoglycans. Mature HAPLN4 is 383 amino acids (aa) in length. It contains one lg-like C2-type domain (aa 46-161) and two link domains (aa 163-365). One potential alternate start site exists 36 aa upstream of the standard start site. Human and mouse HAPLN4 share 91% aa sequence identity.

Long Name
Hyaluronan and Proteoglycan Link Protein 4
Entrez Gene IDs
404037 (Human); 330790 (Mouse)
Alternate Names
BRAL2; BRAL2KIAA1926Brain link protein 2; HAPLN4; hyaluronan and proteoglycan link protein 4; Lpr4

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Citations for Human HAPLN4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Assessment of Possible Contributions of Hyaluronan and Proteoglycan Binding Link Protein 4 to Differential Perineuronal Net Formation at the Calyx of Held
    Authors: Kojiro Nojima, Haruko Miyazaki, Tetsuya Hori, Lydia Vargova, Toshitaka Oohashi
    Frontiers in Cell and Developmental Biology
  2. A peptide for targeted, systemic delivery of imaging and therapeutic compounds into acute brain injuries
    Authors: Aman P. Mann, Pablo Scodeller, Sazid Hussain, Jinmyoung Joo, Ester Kwon, Gary B. Braun et al.
    Nature Communications
  3. Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets
    Authors: M Sonntag, M Blosa, S Schmidt, K Reimann, K Blum, T Eckrich, G Seeger, D Hecker, B Schick, T Arendt, J Engel, M Morawski
    BMC Biol., 2018-09-26;16(1):99.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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