Human HAX-1 Antibody Summary
Ser2-Leu261
Accession # O00165
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human HAX‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HAX-1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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HAX‑1 in HepG2 Human Cell Line. HAX-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAX-1
HAX-1 (HCLS1-associated protein X-1; also HS1-binding protein1) is a 34‑35 kDa intracellular protein with structural similarity to Bcl-2 family proteins. It is ubiquitously expressed, and participates in a variety of cell-specific functions. HAX‑1 blocks cardiomyocyte apoptosis via caspase‑9 inhibition, downregulates conjugated bile acid secretion via BSEP binding, and via PKD2, transmitts integrin signals to the ER. Human HAX-1 is 279 amino acids (aa) in length and contains two Bcl-2 homology regions (aa 37‑56 and 74‑89), a PEST domain (aa 104‑117) and a potential transmembrane segment (aa 261‑279). HS-1 is found mostly in the mitochondria. HAX-1 is also cleaved by caspase-3 at Asp127. Over aa 2‑261, human HAX-1 shares 81% aa identity with mouse HAX-1.
Product Datasheets
Citations for Human HAX-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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The type III secretion system effector EspO of enterohaemorrhagic Escherichia coli inhibits apoptosis through an interaction with HAX ‐1
Authors: Sharanya Chatterjee, Sujinna Lekmeechai, Nicolas Constantinou, Ewa A. Grzybowska, Zuzanna Kozik, Jyoti S. Choudhary et al.
Cellular Microbiology
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PELO negatively regulates HER receptor signalling and metastasis.
Authors: Pedersen K, Canals F, Prat A, Tabernero J, Arribas J
Oncogene, 2013-02-25;33(9):1190-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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