Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody

Catalog #: MAB6487
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6487
MAB6487-SP

Detection of Human Hematopoietic Prostaglandin D Synthase/HPGDS by Western Blot.

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All Hematopoietic Prostaglandin D Synthase/HPGDS Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody Summary

Species Reactivity

Human

Specificity

Detects human Hematopoietic Prostaglandin D Synthase/HPGDS in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 735301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Hematopoietic Prostaglandin D Synthase/HPGDS
Pro2-Leu199
Accession # O60760

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Hematopoietic Prostaglandin D Synthase/HPGDS by Western Blot. Western blot shows lysates of NCI-H460 human large cell lung carcinoma cell line, human lung tissue, and human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Hematopoietic Prostaglandin D Synthase/HPGDS Monoclonal Antibody (Catalog # MAB6487) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hematopoietic Prostaglandin D Synthase/HPGDS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Hematopoietic Prostaglandin D Synthase/HPGDS in HEL 92.1.7 Human Cell Line. Hematopoietic Prostaglandin D Synthase/HPGDS was detected in immersion fixed HEL 92.1.7 human erythroleukemic cell line using Mouse Anti-Human Hematopoietic Prostaglandin D Synthase/HPGDS Monoclonal Antibody (Catalog # MAB6487) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hematopoietic Prostaglandin D Synthase/HPGDS

Prostaglandin D Synthase (PGDS) catalyzes the conversion of prostaglandin (PG) H₂ to PGD₂, which is a major prostanoid produced in a variety of tissues. Two types of PGDS have been isolated; the glutathione-dependent hematopoietic PGDS (HPGDS) and the glutathione-independent lipocalin‑type PGDS (1). HPGDS is a cytosolic enzyme that is expressed in mast cells and antigen presenting cells (2, 3). It is the only mammalian member of the class Sigma glutathione S‑transferase, showing a broad specificity towards standard transferase substrates (4). The PGD₂ produced by HPGDS is involved in many physiological processes such as maintaining body temperature, promotion of sleep, inhibition of platelet aggregation and bronchoconstriction (5). It also functions in immune response and acts as a mediator in allergy and inflammation (6). HPGDS‑specific inhibitors may be therapeutically useful anti‑allergic and anti‑inflammatory drugs.

References

Urade, Y. and Eguchi, N. (2002) Prostaglandins Other Lipid Mediat. 68:375.
Urade, Y. et al. (1990) J. Biol. Chem. 265:371.
Urade, Y. et al. (1989) J. Immunol. 143:2982.
Jowsey, I. R. et al. (2001) Biochem. J. 359:507.
Kanaoka, Y. and Urade, Y. (2003) Prostaglandins Leukot. Essent. Fatty Acids 69:163.
Oguma, T. et al. (2008) Allergol. Int. 57:307.

Entrez Gene IDs

27306 (Human); 54486 (Mouse); 58962 (Rat)

Alternate Names

EC 2.5.1.18; EC 5.3.99.2; glutathione S-transferase sigma; Glutathione S-transferase; Glutathione-dependent PGD synthase; Glutathione-requiring prostaglandin D synthase; GST class-sigma; GSTS; GSTShematopoietic prostaglandin D2 synthase; hematopoietic prostaglandin D synthase; HPGDS; H-PGDS; PGDS; PGDSglutathione-dependent PGD synthetase; Prostaglandin-H2 D-isomerase; PTGDS2

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Citation for Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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