Human Hepassocin/FGL1 Antibody

Catalog #: MAB112302 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB112302-100
MAB112302-SP

Detection of Hepassocin/FGL1 in Human Liver.

2 Images

Product Details

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Hepassocin/FGL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Hepassocin/FGL1 in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1052936

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human liver.

Immunocytochemistry

8-25 µg/mL

Immersion fixed HepG2 human hepatocellular carcinoma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Detection of Hepassocin/FGL1 in Human Liver. Hepassocin/FGL1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB112302) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry

View Larger

Detection of Hepassocin/FGL1 in HepG2 Human hepatocellular carcinoma cell line (positive) and K562 Human chronic myelogenous leukemia cell line (negative). Hepassocin/FGL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative) using Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB112302) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and FGL1, is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. Hepassocin may play a role in the development of hepatocellular carcinomas.

Long Name

Fibrinogen Like 1

Entrez Gene IDs

2267 (Human); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Alternate Names

FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1