Human Hexokinase 1/2 Antibody Summary
Phe11-Arg917
Accession # P52789
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Hexokinase 1/2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Hexokinase 1/2 Monoclonal Antibody (Catalog # MAB8179) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Hexokinase 1/2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Hexokinase 1/2 in Human Cervical Cancer Tissue. Hexokinase 1/2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human Hexokinase 1/2 Monoclonal Antibody (Catalog # MAB8179) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Hexokinase 1/2 by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Hexokinase 1/2 at approximately 96 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Hexokinase 1/2 Monoclonal Antibody (Catalog # MAB8179). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hexokinase 1/2
Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step in hexose metabolism (1). Phosphorylation of a hexose adds charge to molecule thereby making it difficult to transport out of a cell. The hexose is therefore retained for intracellular metabolic processes, such as glycolysis or glycogen synthesis. In most organisms, glucose is the most important substrate of hexokinases and glucose-6-phosphate is the most important product. There are four mammalian hexokinases (2). Hexokinase 1, 2 and 3 are referred to as high-affinity hexokinases because their Km for glucose is below 1 mM. Hexokinase 4 is specific for glucose and is also referred to as glucokinase (3). Hexokinase 2 (HK2), also known as muscle form hexokinase, localizes to the outer membrane of mitochondria and is present in adipose tissue, skeletal muscle, and heart (4). The amino acids corresponding to the mitochondrial binding domain (5) have been removed in the recombinant enzyme. Like Hexokinase 1 (HK1), HK2 contains two homologous halves that may have evolved from an ancestral hexokinase through gene duplication and tandem ligation (6). Unlike HK1, in HK2 both the C-terminal and N-terminal portions are catalytically active with the N-terminal half having higher activity than the C-terminal half (7). In HK2 both the N-terminal and C-terminal halves exhibit product inhibition. HK2 overexpression is required for tumor growth making HK2 an attractive oncotarget (4). The enzymatic activity of recombinant human HK2 is measured using a phosphatase-coupled method (8).
- Aleshin, A.E. et al. (1998) Structure 6:39-50
- Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
- Lange, A.J. et al. (1991) Biochem. J. 277: 159.
- Patra, K.C. et al. (2013) Cancer Cell. 24:213.
- Bianchi M et al. (1998) Mol Cell Biochem 189:185.
- Ureta, T. (1982) Comp Biochem Physiol. B. 71B:549.
- Ahn, K.J. et al. (2009) BMB Rep. 42:350.
- Wu, Z.L. (2011) PLoS One 6:e23172.
Product Datasheets
Citations for Human Hexokinase 1/2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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GIV/Girdin, a non-receptor modulator for Galphai/s, regulates spatiotemporal signaling during sperm capacitation and is required for male fertility
Authors: S Reynoso, V Castillo, GD Katkar, I Lopez-Sanc, S Taheri, C Espinoza, C Rohena, D Sahoo, P Gagneux, P Ghosh
Elife, 2021-08-19;10(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Metformin suppresses Nrf2-mediated chemoresistance in hepatocellular carcinoma cells by increasing glycolysis
Authors: Liangyu Cai, Xin Jin, Jiannan Zhang, Le Li, Jinfeng Zhao
Aging (Albany NY)
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