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Human HGF Quantikine ELISA Kit

Catalog #: DHG00
92 Citations 2 Reviews Datasheet / COA / SDS
Newer Version Available: DHG00B
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DHG00 has been discontinued and is replaced by DHG00B.

Human HGF ELISA Standard Curve
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Citations (92)
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Reviews (2)
Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human HGF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.25 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Citrate Plasma (50 uL)
Sensitivity
40 pg/mL
Assay Range
125.0 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant human HGF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human HGF in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum (n=60)1257671-1992289
EDTA plasma (n=60)787469-113136
Citrate plasma (n=71)440251-742102

Cell Culture Supernates - Human peripheral blood mononuclear cells (5 x 106 cells/mL) were cultured in RPMI supplemented with 5% fetal calf serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human HGF.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
UnstimulatedND548
Stimulated152145
ND=Non-detectable


Product Summary

The Quantikine Human HGF Immunoassay is a 4.25 or 4.5 hour solid phase ELISA that is designed to measure HGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human pro-HGF and antibodies raised against the recombinant factor and has been shown to quantitate recombinant human HGF accurately. Results obtained using natural HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 2 3
n 20 20 40 40
Mean (pg/mL) 425 991 1058 4058
Standard Deviation 30.2 43.2 75.1 264
CV% 7.1 4.4 7.1 6.5

Serum, EDTA Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 2 3 1 2 3
n 20 20 40 40 40
Mean (pg/mL) 999 3759 363 967 3790
Standard Deviation 56.2 154 25.8 81.2 205
CV% 5.6 4.1 7.1 8.4 5.4

Recovery

The recovery of HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 92 86-101
Citrate Plasma (n=5) 105 99-114
EDTA Plasma (n=5) 100 85-111
Serum (n=5) 99 91-108

Linearity

To assess the linearity of the assay, five samples of each sample type were spiked with high concentrations of HGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human HGF Linearity
Human HGF ELISA Linearity

Scientific Data

N/A Human HGF ELISA Standard Curve View Larger

Human HGF ELISA Standard Curve

N/A Human HGF ELISA Cell Culture Supernate Standard Curve View Larger

Human HGF ELISA Cell Culture Supernate Standard Curve

N/A Human HGF ELISA Serum/Plasma Standard Curve View Larger

Human HGF ELISA Serum/Plasma Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGF

HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.

Long Name:
Hepatocyte Growth Factor
Entrez Gene IDs:
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
Alternate Names:
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 150 µL Assay Diluent
  3.   Add 150 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well.
  7.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1.75 hours.
  8.   Aspirate and wash 4 times.
    9. 200 µL Substrate Solution
  9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human HGF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

92 Citations: Showing 1 - 10
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  1. Rapid and effective preparation of clonal bone marrow-derived mesenchymal stem/stromal cell sheets to reduce renal fibrosis
    Authors: S Kameishi, CM Dunn, M Oka, K Kim, YK Cho, SU Song, DW Grainger, T Okano
    Scientific Reports, 2023-03-17;13(1):4421.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Novel rAAV vector mediated intrathecal HGF delivery has an impact on neuroimmune modulation in the ALS motor cortex with TDP-43 pathology
    Authors: B Genç, B Nho, H Seung, B Helmold, H Park, Ö Gözütok, S Kim, J Park, S Ye, H Lee, N Lee, SS Yu, S Kim, J Lee, H Özdinler
    Gene Therapy, 2023-02-24;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Rilotumumab Resistance Acquired by Intracrine Hepatocyte Growth Factor Signaling
    Authors: F Cecchi, K Rex, J Schmidt, CD Vocke, YH Lee, S Burkett, D Baker, MA Damore, A Coxon, TL Burgess, DP Bottaro
    Cancers, 2023-01-11;15(2):.
    Species: Xenograft
    Sample Types: Serum
  4. ARHGAP-RhoA signaling provokes homotypic adhesion-triggered cell death of metastasized diffuse-type gastric cancer
    Authors: M Komatsu, H Ichikawa, F Chiwaki, H Sakamoto, R Komatsuzak, M Asaumi, K Tsunoyama, T Fukagawa, H Matsushita, N Boku, K Matsusaki, F Takeshita, T Yoshida, H Sasaki
    Oncogene, 2022-09-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Mesenchymal Stem Cell Sheet Centrifuge-Assisted Layering Augments Pro-Regenerative Cytokine Production
    Authors: S Bou-Ghanna, K Kim, M Kondo, DW Grainger, T Okano
    Cells, 2022-09-12;11(18):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells
    Authors: N Igarashi, K Miyata, TM Loo, M Chiba, A Hanyu, M Nishio, H Kawasaki, H Zheng, S Toyokuni, S Kon, K Moriyama, Y Fujita, A Takahashi
    Oncogene, 2022-07-18;13(1):4157.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Cytokine response over the course of COVID-19 infection in pregnant women
    Authors: DB Rosen, EA Murphy, RS Gejman, A Capili, RL Friedlande, S Rand, KA Cagino, SM Glynn, KC Matthews, JM Kubiak, J Yee, M Prabhu, LE Riley, YJ Yang
    Cytokine, 2022-04-25;154(0):155894.
    Species: Human
    Sample Types: Serum
  8. Combination Therapy of Placenta-Derived Mesenchymal Stem Cells with WKYMVm Promotes Hepatic Function in a Rat Model with Hepatic Disease via Vascular Remodeling
    Authors: JH Jun, S Park, JY Kim, JY Lim, GT Park, JH Kim, GJ Kim
    Cells, 2022-01-11;11(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Freezing of cell sheets using a 3D freezer produces high cell viability after thawing
    Authors: K Ueno, S Ike, N Yamamoto, Y Matsuno, H Kurazumi, R Suzuki, S Katsura, B Shirasawa, K Hamano
    Biochemistry and Biophysics Reports, 2021-11-08;28(0):101169.
    Species: Human
    Sample Types: Cell culture supernate
  10. HGF/MET in osteogenic differentiation of primary human palatal periosteum-derived mesenchymal stem cells
    Authors: NY Naung, WJ Duncan, RK De Silva, DE Coates
    Journal of oral science, 2021-09-15;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Adipose-derived mesenchymal stem cells preserve cardiac function via ANT-1 in dilated cardiomyopathy hamster model
    Authors: D Mori, S Miyagawa, T Kido, H Hata, T Ueno, K Toda, T Kuratani, M Oota, K Kawai, H Kurata, H Nishida, Y Sawa
    Regenerative Therapy, 2021-07-09;18(0):182-190.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Defined MSC exosome with high yield and purity to improve regenerative activity
    Authors: JY Kim, WK Rhim, YI Yoo, DS Kim, KW Ko, Y Heo, CG Park, DK Han
    Journal of tissue engineering, 2021-04-20;12(0):2041731421100.
    Species: Human
    Sample Types: Cell Lysates
  13. Suppression of Peritoneal Fibrosis by Sonoporation of Hepatocyte Growth Factor Gene-Encoding Plasmid DNA in Mice
    Authors: K Nishimura, K Ogawa, M Kawaguchi, S Fumoto, H Mukai, S Kawakami
    Pharmaceutics, 2021-01-18;13(1):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Crosstalk between Mast Cells and Lung Fibroblasts Is Modified by Alveolar Extracellular Matrix and Influences Epithelial Migration
    Authors: M Bagher, O Rosmark, L Elowsson R, A Nybom, S Wasserstro, C Müller, XH Zhou, G Dellgren, O Hallgren, L Bjermer, AK Larsson-Ca, G Westergren
    International Journal of Molecular Sciences, 2021-01-06;22(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Therapeutic Angiogenesis by a "Dynamic Duo": Simultaneous Expression of HGF and VEGF165 by Novel Bicistronic Plasmid Restores Blood Flow in Ischemic Skeletal Muscle
    Authors: E Slobodkina, M Boldyreva, M Karagyaur, R Eremichev, N Alexandrus, V Balabanyan, Z Akopyan, Y Parfyonova, V Tkachuk, P Makarevich
    Pharmaceutics, 2020-12-18;12(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Peritumoral activated hepatic stellate cells are associated with hepatic recurrence for resectable colorectal adenocarcinoma liver metastasis following resection
    Authors: L Deng, T Li, Y Liao, S Liu, Z Xie, Z Huang, H Dai, J Li, X Lei
    Oncol Lett, 2020-09-23;20(6):287.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Autocrine HGF/c-Met signaling pathway confers aggressiveness in lymph node adult T-cell leukemia/lymphoma
    Authors: H Totani, K Shinjo, M Suzuki, K Katsushima, S Mase, A Masaki, A Ito, M Ri, S Kusumoto, H Komatsu, T Ishida, H Inagaki, S Iida, Y Kondo
    Oncogene, 2020-08-04;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates, Serum
  18. Repeated Freezing Procedures Preserve Structural and Functional Properties of Amniotic Membrane for Application in Ophthalmology
    Authors: O Pogozhykh, N Hofmann, O Gryshkov, C von Kaisen, M Mueller, B Glasmacher, D Pogozhykh, M Börgel, R Blasczyk, C Figueiredo
    Int J Mol Sci, 2020-06-04;21(11):.
    Species: Human
    Sample Types: Tissue Homogenates
  19. Detection of hepatocyte growth factor in oral rinses using water for possible periodontal diagnosis
    Authors: S Suzuki, A Aoki, S Katagiri, S Maekawa, K Ejiri, S Kong, M Nagata, Y Yamaguchi, M Ohshima, Y Izumi
    J Oral Sci, 2020-05-15;0(0):.
    Species: Human
    Sample Types: Oral Rinse
  20. Aberrant activation of hepatocyte growth factor/MET signaling promotes beta-catenin-mediated prostatic tumorigenesis
    Authors: J Aldahl, J Mi, A Pineda, WK Kim, A Olson, E Hooker, Y He, EJ Yu, V Le, DH Lee, J Geradts, Z Sun
    J. Biol. Chem., 2019-12-09;295(2):631-644.
    Species: Mouse
    Sample Types: Serum
  21. Hypoxic Preconditioning Enhances Survival and Proangiogenic Capacity of Human First Trimester Chorionic Villus-Derived Mesenchymal Stem Cells for Fetal Tissue Engineering
    Authors: D Hao, C He, B Ma, L Lankford, L Reynaga, DL Farmer, F Guo, A Wang
    Stem Cells Int, 2019-11-12;2019(0):9695239.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Human mesenchymal stem cell sheets in xeno-free media for possible allogenic applications
    Authors: K Kim, S Bou-Ghanna, H Thorp, DW Grainger, T Okano
    Sci Rep, 2019-10-08;9(1):14415.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Bioinspired Self-assembling Peptide Hydrogel with Proteoglycan-assisted Growth Factor Delivery for Therapeutic Angiogenesis
    Authors: LC Huang, HC Wang, LH Chen, CY Ho, PH Hsieh, MY Huang, HC Wu, TW Wang
    Theranostics, 2019-09-21;9(23):7072-7087.
    Species: Human
    Sample Types: Hydrogel Supernates
  24. Extracellular lipid loading augments hypoxic paracrine signaling and promotes glioma angiogenesis and macrophage infiltration
    Authors: S Offer, JA Menard, JE Pérez, KG de Oliveir, VI Chandran, MC Johansson, A Bång-Ruden, P Siesjö, A Ebbesson, I Hedenfalk, PC Sundgren, A Darabi, M Belting
    J. Exp. Clin. Cancer Res., 2019-06-07;38(1):241.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins
    Authors: M Nakao, D Inanaga, K Nagase, H Kanazawa
    Regen Ther, 2019-05-10;11(0):34-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Increased hepatocyte growth factor levels over 2 years are associated with coronary heart disease to Increased hepatocyte growth factor levels over 2 years are associated with coronary heart disease: the Multi-Ethnic Study of Atherosclerosis (MESA)
    Authors: PA Decker, NB Larson, EJ Bell, JS Pankow, NQ Hanson, CL Wassel, MY Tsai, SJ Bielinski
    Am. Heart J., 2019-04-13;213(0):30-34.
    Species: Human
    Sample Types: Serum
  27. Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches
    Authors: C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K Rouger
    Stem Cell Res Ther, 2018-05-02;9(1):128.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Forty-nine gastric cancer cell lines with integrative genomic profiling for development of c-MET inhibitor
    Authors: HJ Kim, SK Kang, WS Kwon, TS Kim, I Jeong, HC Jeung, M Kragh, ID Horak, HC Chung, SY Rha
    Int. J. Cancer, 2018-02-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  29. Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-?B signaling pathway in vivo and in vitro
    Authors: B Liu, F Ding, D Hu, Y Zhou, C Long, L Shen, Y Zhang, D Zhang, G Wei
    Stem Cell Res Ther, 2018-01-12;9(1):7.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. miRNA profiling of NurOwn�: mesenchymal stem cells secreting neurotrophic factors
    Authors: Y Gothelf, H Kaspi, N Abramov, R Aricha
    Stem Cell Res Ther, 2017-11-07;8(1):249.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Exogenous growth factors bFGF, EGF and HGF do not influence viability and phenotype of V600EBRAF melanoma cells and their response to vemurafenib and trametinib in vitro
    Authors: I Zalesna, M Osrodek, ML Hartman, M Rozanski, M Sztiller-S, K Niewinna, D Nejc, M Czyz
    PLoS ONE, 2017-08-22;12(8):e0183498.
    Species: Human
    Sample Types: Whole Cells
  32. Human hepatocyte growth factor inhibits early neointima formation in rabbit abdominal aortae following ultrasound-guided balloon injury
    Authors: L Mei, Y He, H Wang, Y Jin, S Wang, C Jin
    Mol Med Rep, 2017-08-11;0(0):.
    Species: Rabbit
    Sample Types: Tissue Homogenates
  33. Five-weekly S-1 plus cisplatin therapy combined with trastuzumab therapy in HER2-positive gastric cancer: a phase II trial and biomarker study (WJOG7212G)
    Authors: Y Miura, Y Sukawa, S Hironaka, M Mori, K Nishikawa, S Tokunaga, H Okuda, T Sakamoto, K Taku, K Nishikawa, T Moriwaki, Y Negoro, Y Kimura, K Uchino, K Shinozaki, H Shinozaki, N Musha, H Yoshiyama, T Tsuda, Y Miyata, N Sugimoto, T Shirakawa, M Ito, K Yonesaka, K Yoshimura, N Boku, K Nosho, T Takano, I Hyodo
    Gastric Cancer, 2017-05-11;0(0):.
    Species: Human
    Sample Types: Serum
  34. Comparison of fibrin glue and Vicryl sutures in conjunctival autografting for pterygium surgery
    Authors: X Wang, Y Zhang, L Zhou, R Wei, L Dong
    Mol. Vis., 2017-04-19;23(0):275-285.
    Species: Human
    Sample Types: Tears
  35. Preclinical development of a humanized neutralizing antibody targeting HGF
    Authors: H Kim, SH Hong, JY Kim, IC Kim, YW Park, SJ Lee, SW Song, JJ Kim, G Park, TM Kim, YH Kim, JB Park, J Chung, IH Kim
    Exp. Mol. Med, 2017-03-24;49(3):e309.
    Species: Human
    Sample Types: Serum
  36. Hypoxic preconditioning of human cardiosphere-derived cell sheets enhances cellular functions via activation of the PI3K/Akt/mTOR/HIF-1? pathway
    Authors: Y Tanaka, T Hosoyama, A Mikamo, H Kurazumi, A Nishimoto, K Ueno, B Shirasawa, K Hamano
    Am J Transl Res, 2017-02-15;9(2):664-673.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes
    PLoS ONE, 2016-12-19;11(12):e0168058.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Erythropoietin induces production of hepatocyte growth factor from bone marrow mesenchymal stem cells in�vitro
    Authors: Majid Mossahebi-
    Biologicals, 2016-11-16;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia
    Stem Cell Res Ther, 2016-09-09;7(1):128.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
    Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
    J Am Heart Assoc, 2016-04-18;5(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. M-CSF in a new biomarker panel with HE4 and CA 125 in the diagnostics of epithelial ovarian cancer patients.
    Authors: Bedkowska G, Lawicki S, Gacuta E, Pawlowski P, Szmitkowski M
    J Ovarian Res, 2015-05-03;8(0):27.
    Species: Human
    Sample Types: Plasma
  42. The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.
    Authors: Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor A, Yasuda Y, Niki T
    BMC Cancer, 2015-02-25;15(0):82.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Multiple regulatory mechanisms of hepatocyte growth factor expression in malignant cells with a short poly(dA) sequence in the HGF gene promoter.
    Authors: Sakai K, Takeda M, Okamoto I, Nakagawa K, Nishio K
    Oncol Lett, 2014-11-11;9(1):405-410.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Diversity of biological effects induced by longwave UVA rays (UVA1) in reconstructed skin.
    Authors: Marionnet C, Pierrard C, Golebiewski C, Bernerd F
    PLoS ONE, 2014-08-20;9(8):e105263.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma.
    Authors: Imura, Yoshinor, Yasui, Hirohiko, Outani, Hidetats, Wakamatsu, Toru, Hamada, Kenichir, Nakai, Takaaki, Yamada, Shutaro, Myoui, Akira, Araki, Nobuhito, Ueda, Takafumi, Itoh, Kazuyuki, Yoshikawa, Hideki, Naka, Norifumi
    Mol Cancer, 2014-08-07;13(0):185.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Serum
  46. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
    Authors: Chou M, Burnouf T, Wang T
    PLoS ONE, 2014-06-19;9(6):e99145.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Establishment of a novel clear cell sarcoma cell line (Hewga-CCS), and investigation of the antitumor effects of pazopanib on Hewga-CCS.
    Authors: Outani, Hidetats, Tanaka, Takaaki, Wakamatsu, Toru, Imura, Yoshinor, Hamada, Kenichir, Araki, Nobuhito, Itoh, Kazuyuki, Yoshikawa, Hideki, Naka, Norifumi
    BMC Cancer, 2014-06-19;14(0):455.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer.
    Authors: Takahashi, N, Yamada, Y, Furuta, K, Honma, Y, Iwasa, S, Takashima, A, Kato, K, Hamaguchi, T, Shimada, Y
    Br J Cancer, 2014-05-06;110(11):2716-27.
    Species: Human
    Sample Types: Serum
  49. Effects of high intensity training and high volume training on endothelial microparticles and angiogenic growth factors.
    Authors: Wahl P, Jansen F, Achtzehn S, Schmitz T, Bloch W, Mester J, Werner N
    PLoS ONE, 2014-04-25;9(4):e96024.
    Species: Human
    Sample Types: Serum
  50. The molecular mechanism underlying the proliferating and preconditioning effect of vitamin C on adipose-derived stem cells.
    Authors: Kim, Ji Hye, Kim, Wang-Kyu, Sung, Young Kw, Kwack, Mi Hee, Song, Seung Yo, Choi, Joon-Seo, Park, Sang Gyu, Yi, TacGhee, Lee, Hyun-Joo, Kim, Dae-Duk, Seo, Hyun Min, Song, Sun U, Sung, Jong-Hyu
    Stem Cells Dev, 2014-03-21;23(12):1364-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. In vitro hepatic trans-differentiation of human mesenchymal stem cells using sera from congestive/ischemic liver during cardiac failure.
    Authors: Bishi, Dillip K, Mathapati, Santosh, Cherian, Kotturat, Guhathakurta, Soma, Verma, Rama Sha
    PLoS ONE, 2014-03-18;9(3):e92397.
    Species: Human
    Sample Types: Serum
  52. A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases.
    Authors: Tabernero J, Elez M, Herranz M, Rico I, Prudkin L, Andreu J, Mateos J, Carreras M, Han M, Gifford J, Credi M, Yin W, Agarwal S, Komarnitsky P, Baselga J
    Clin Cancer Res, 2014-03-14;20(10):2793-804.
    Species: Human
    Sample Types: Serum
  53. Gene expression profiling and secretome analysis differentiate adult-derived human liver stem/progenitor cells and human hepatic stellate cells.
    Authors: Berardis S, Lombard C, Evraerts J, El Taghdouini A, Rosseels V, Sancho-Bru P, Lozano J, van Grunsven L, Sokal E, Najimi M
    PLoS ONE, 2014-01-21;9(1):e86137.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Analysis of plasma cytokines and angiogenic factors in patients with pretreated urothelial cancer receiving Pazopanib: the role of circulating interleukin-8 to enhance the prognostic accuracy.
    Authors: Necchi A, Pennati M, Zaffaroni N, Landoni E, Giannatempo P, Raggi D, Schwartz L, Morosi C, Crippa F, Fare E, Nicolai N, Lanocita R, Sava T, Sacco C, Messina C, Ortega C, De Braud F, Salvioni R, Daidone M, Gianni A, Mariani L
    Br J Cancer, 2013-11-14;110(1):26-33.
    Species: Human
    Sample Types: Plasma
  55. Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia.
    Authors: Conforti A, Biagini S, Del Bufalo F, Sirleto P, Angioni A, Starc N, Li Pira G, Moretta F, Proia A, Contoli B, Genovese S, Ciardi C, Avanzini M, Rosti V, Lo-Coco F, Locatelli F, Bernardo M
    PLoS ONE, 2013-11-07;8(11):e76989.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro.
    Authors: Li Q, Zhang A, Tao C, Li X, Jin P
    Biochem Biophys Res Commun, 2013-10-30;441(3):675-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
    Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
    BMC Cell Biol, 2013-10-30;14(0):48.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Clinical outcome, proteome kinetics and angiogenic factors in serum after thermoablation of colorectal liver metastases.
    Authors: Wertenbroek, Marieke, Schepers, Marianne, Kamminga-Rasker, Hannetta, Bottema, Jan T, Muller Kobold, Anneke C, Roelofsen, Han, de Jong, Koert P
    BMC Cancer, 2013-05-30;13(1):266.
    Species: Human
    Sample Types: Serum
  59. Hepatocyte growth factor pathway upregulation in the bone marrow microenvironment in multiple myeloma is associated with lytic bone disease.
    Authors: Kristensen I, Christensen J, Lyng M, Moller M, Pedersen L, Rasmussen L, Ditzel H, Abildgaard N
    Br J Haematol, 2013-02-22;161(3):373-82.
    Species: Human
    Sample Types: Plasma
  60. Measuring urinary tubular biomarkers in type 2 diabetes does not add prognostic value beyond established risk factors.
    Kidney Int, 2012-06-20;82(7):812-8.
    Species: Human
    Sample Types: Serum
  61. Comparison of the levels of the growth factors in umbilical cord serum and human milk and its clinical significance.
    Authors: Patki S, Patki U, Patil R, Indumathi S, Kaingade P, Bulbule A, Nikam A, Pishte A
    Cytokine, 2012-05-19;59(2):305-8.
    Species: Human
    Sample Types: Serum
  62. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
    Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
    Clin. Cancer Res., 2012-02-28;18(8):2290-300.
    Species: Human
    Sample Types: Plasma
  63. The secretion pattern of perivascular fat cells is different from that of subcutaneous and visceral fat cells.
    Authors: Rittig K, Dolderer JH, Balletshofer B, Machann J, Schick F, Meile T, Kuper M, Stock UA, Staiger H, Machicao F, Schaller HE, Konigsrainer A, Haring HU, Siegel-Axel DI
    Diabetologia, 2012-02-13;55(5):1514-25.
    Species: Human
    Sample Types: Serum
  64. Validation and quality control of ELISAs for the use with human saliva samples.
    Authors: Jaedicke KM, Taylor JJ, Preshaw PM
    J. Immunol. Methods, 2012-01-28;377(1):62-5.
    Species: Human
    Sample Types: Saliva
  65. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
    Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
    Blood, 2011-01-21;117(13):3485-93.
    Species: Human
    Sample Types: Plasma
  66. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
    Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
    ALTEX, 2011-01-01;28(4):305-16.
    Species: Human
    Sample Types: Cell Lysates
  67. Human stem/progenitor cells from bone marrow enhance glial differentiation of rat neural stem cells: a role for transforming growth factor beta and Notch signaling.
    Authors: Robinson AP, Foraker JE, Ylostalo J, Prockop DJ
    Stem Cells Dev., 2010-09-14;20(2):289-300.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Impact of serum hepatocyte growth factor on treatment response to epidermal growth factor receptor tyrosine kinase inhibitors in patients with non-small cell lung adenocarcinoma.
    Authors: Kasahara K, Arao T, Sakai K, Matsumoto K, Sakai A, Kimura H, Sone T, Horiike A, Nishio M, Ohira T, Ikeda N, Yamanaka T, Saijo N, Nishio K
    Clin. Cancer Res., 2010-08-02;16(18):4616-24.
    Species: Human
    Sample Types: Serum
  69. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
    Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
    Cardiovasc. Res., 2010-07-29;89(1):157-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Wnt activity defines colon cancer stem cells and is regulated by the microenvironment.
    Authors: Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP
    Nat. Cell Biol., 2010-04-25;12(5):468-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Safety, pharmacokinetics, and pharmacodynamics of AMG 102, a fully human hepatocyte growth factor-neutralizing monoclonal antibody, in a first-in-human study of patients with advanced solid tumors.
    Authors: Gordon MS, Sweeney CS, Mendelson DS
    Clin. Cancer Res., 2010-01-12;16(2):699-710.
    Species: Human
    Sample Types: Plasma
  72. Alternative proteolytic processing of hepatocyte growth factor during wound repair.
    Authors: Buchstein N, Hoffmann D, Smola H, Lang S, Paulsson M, Niemann C, Krieg T, Eming SA
    Am. J. Pathol., 2009-04-23;174(6):2116-28.
    Species: Human
    Sample Types: Tissue Secretion
  73. IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism.
    Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
    Stem Cells, 2009-01-01;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
    Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
    Arch. Immunol. Ther. Exp. (Warsz.), 2008-05-30;56(3):207-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. Clinical correlations and prognostic relevance of HGF, VEGF AND FGF expression in Brazilian patients with non-Hodgkin lymphoma.
    Authors: Etto L, Lacerda E, Baiocchi O, Silva V, Dalboni M, Alves A, Silva M, Vettore A, Colleoni G
    Leuk. Lymphoma, 2008-02-01;49(2):257-64.
    Species: Human
    Sample Types: Serum
  76. Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis.
    Authors: Bogatkevich GS, Ludwicka-Bradley A, Highland KB, Hant F, Nietert PJ, Singleton CB, Feghali-Bostwick CA, Silver RM
    Arthritis Rheum., 2007-07-01;56(7):2432-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  77. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007-04-19;25(8):1862-71.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Hepatocyte growth factor levels in gingival crevicular fluid in health, disease, and after treatment.
    Authors: Nagaraja C, Pradeep AR
    J. Periodontol., 2007-04-01;78(4):742-7.
    Species: Human
    Sample Types:
  79. A fusion protein of hepatocyte growth factor for immobilization to collagen.
    Authors: Kitajima T, Terai H, Ito Y
    Biomaterials, 2007-01-19;28(11):1989-97.
    Species: Human
    Sample Types: Recombinant Protein
  80. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
    Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
    Am. J. Pathol., 2006-11-01;169(5):1767-83.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Humoral and cellular factors responsible for coronary collateral formation.
    Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
    Am. J. Cardiol., 2006-08-31;98(9):1194-7.
    Species: Human
    Sample Types: Plasma
  82. Relation of various plasma growth factor levels in patients with stable angina pectoris and total occlusion of a coronary artery to the degree of coronary collaterals.
    Authors: Briguori C, Testa U, Colombo A, Petrucci E, Condorelli G, Airoldi F, Peschle C, Condorelli G
    Am. J. Cardiol., 2005-12-20;97(4):472-6.
    Species: Human
    Sample Types: Serum
  83. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005-11-01;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. Exercise acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells.
    Authors: Rehman J, Li J, Parvathaneni L, Karlsson G, Panchal VR, Temm CJ, Mahenthiran J, March KL
    J. Am. Coll. Cardiol., 2004-06-16;43(12):2314-8.
    Species: Human
    Sample Types: Plasma
  85. Differential macrophage infiltration in early and advanced endometriosis and adjacent peritoneum.
    Authors: Khan KN, Masuzaki H, Fujishita A, Kitajima M, Sekine I, Ishimaru T
    Fertil. Steril., 2004-03-01;81(3):652-61.
    Species: Human
    Sample Types: Cell Culture Supernates
  86. Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
    Authors: Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H
    J Trauma, 2004-02-01;56(2):259-64.
    Species: Human
    Sample Types: Plasma
  87. Hepatocyte growth factor and its role in the pathogenesis of retinal detachment.
    Authors: Jin M, Chen Y, He S, Ryan SJ, Hinton DR
    Invest. Ophthalmol. Vis. Sci., 2004-01-01;45(1):323-9.
    Species: Rabbit
    Sample Types: Vitreous Humor
  88. Hepatocyte growth factor in the vitreous fluid of patients with proliferative diabetic retinopathy: its relationship with vascular endothelial growth factor and retinopathy activity.
    Authors: Simo R, Lecube A, Garcia-Arumi J, Carrasco E, Hernandez C
    Diabetes Care, 2004-01-01;27(1):287-8.
    Species: Human
    Sample Types: Vitreous Humor
  89. Hepatocyte growth factor (HGF) system in gingiva: HGF activator expression by gingival epithelial cells.
    Authors: Ohshima M, ù °, Sakai A, Sawamoto Y, Seki K, Ito K, Otsuka K
    2002-12-01;44(3):129-34.
    Species: Human
    Sample Types:
  90. Association of interleukin-6 and estradiol with hepatocyte growth factor in peritoneal fluid of women with endometriosis.
    Authors: Khan KN, Masuzaki H, Fujishita A, Hamasaki T, Kitajima M, Hasuo A, Miyamura Y, Ishimaru T
    Acta Obstet Gynecol Scand, 2002-08-01;81(8):764-71.
    Species: Human
    Sample Types: Peritoneal Fluid
  91. Selective plasma filtration for treatment of fulminant hepatic failure induced by D-galactosamine in a pig model.
    Authors: Ho DW, Fan ST, To J, Woo YH, Zhang Z, Lau C, Wong J
    Gut, 2002-06-01;50(6):869-76.
    Species: Porcine
    Sample Types: Plasma
  92. Exhaled breath condensate and serum levels of hepatocyte growth factor in pneumonia.
    Authors: Nayeri F, Millinger E, Nilsson I, Zetterström, Brudin L, Forsberg P
    Respir Med, 2002-02-01;96(2):115-9.
    Species: Human
    Sample Types: Exhaled Breath Condensate (EBC)

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Human HGF Quantikine ELISA Kit
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Sample Tested: Serum
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Sample Tested: Cell culture supernatant
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