Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

Name: Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
Brand: R&D Systems
SKU: PP-N3224-00
Rating: 5 (1 reviews)

Catalog #: PP-N3224-00
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
PP-N3224-00

HNF‑4 gamma /NR2A2 in Rat Small Intestine.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HNF-4 gamma/NR2A2 MAb (Clone N3224) Summary

Species Reactivity

Human

Source

Monoclonal Mouse IgG2a Clone # N3224

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Please refer to the PDF datasheet for specifications.

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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HNF‑4 gamma /NR2A2 in Rat Small Intestine. HNF‑4 gamma /NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF‑4 gamma /NR2A2 Monoclonal Antibody (Catalog # PP‑N3224‑00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HNF-4 gamma/NR2A2

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

Long Name

Hepatocyte Nuclear Factor 4 gamma

Entrez Gene IDs

3174 (Human)

Alternate Names

hepatocyte nuclear factor 4, gamma; hepatocyte nuclear factor 4-gamma; HNF4 gamma; HNF-4 gamma; HNF4G; HNF-4-gamma; NR2A2; NR2A2Nuclear receptor subfamily 2 group A member 2; NR2A3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Steroid Hormone Receptors and Regulators

Citation for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

HNF4G increases cisplatin resistance in lung adenocarcinoma via the MAPK6/Akt pathway
Authors: J Liang, G Zhao, Y Bian, G Bi, Q Sui, H Zhang, H Shi, G Shan, Y Huang, Z Chen, L Wang, C Zhan
PeerJ, 2023-03-10;11(0):e14996.