Human HOXD10 Antibody

Catalog #: AF10413 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF10413-100
AF10413-SP

HOXD10 in Human iPS Cells Differentiated to NPCs.

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Human HOXD10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HOXD10 in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HOXD10
Arg153-Thr262
Accession # P28358

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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HOXD10 in Human iPS Cells Differentiated to NPCs. HOXD10 was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to neural progenitor cells (NPC) using Goat Anti-Human HOXD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10413) at 10 µg/mL, and co-stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259) at 10 µg/mL, for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001), NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining for HOXD10 was localized to nucleus, while Nestin co-staining was cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOXD10

HOXD10 is a sequence-specific transcription factor that belongs to the Abd-B homeobox family. It is a nuclear protein that is expressed in developing limb buds and is involved in differentiation and limb development. HOXD10 mutations have been associated with foot deformities such as rocker-bottom foot and the deformity seen in Charcot-Marie-Tooth disease. HOXD10 also functions as a candidate tumor suppressor. In gastric cancer there is downregulation in gastric cancer tissues and cell lines relative to normal stomach tissues. It has also been reported to suppress ovarian cancer, hepatocellular carcinoma and other cancer cells. Re-expression of HOXD10 results in significant inhibition of cell survival, induction of cell apoptosis and impairment of cell migration and invasion.

Long Name

Homeobox D10

Entrez Gene IDs

3236 (Human)

Alternate Names

homeo box 4D; homeo box D10; homeobox D10; Homeobox protein Hox-4D; Homeobox protein Hox-4E; homeobox protein Hox-D10; HOX4; Hox-4.4; HOX4D; HOX4DHOX4; HOX4E; HOXD10