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Human Hrk Antibody

Catalog #: AF851
1 Citation Datasheet / COA / SDS

Discontinued Product

AF851 has been discontinued.
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Detection of Human Hrk by Western Blot.
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Citations (1)
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Human Hrk Antibody Summary

Species Reactivity
Human
Specificity
Detects human Hrk in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled human Hrk synthetic peptide
RTMWRRRARSR RAPAPGAALPC
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Hrk antibody by Western Blot. View Larger

Detection of Human Hrk by Western Blot. Western blot shows lysates ofE. coliexpressing recombinant human Hrk-green fluorescent protein fusion. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Hrk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF851) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Hrk was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hrk

Activator of apoptosis Hrk regulates apoptosis through interaction with death-repressor proteins Bcl-2 and Bcl-X(L). The HRK protein lacks significant homology to other BCL2 family members except for an 8-amino acid region that was similar to the BCL2 homology domain-3 (BH3) motif of BIK. HRK interacts with BCL2 and BCLXL via the BH3 domain, but not with the death-promoting BCL2-related proteins BAX, BAK, or BCLXS. HRK localizes to membranes of intracellular organelles in a pattern similar to that previously reported for BCL2 and BCLXL.

Long Name
Harakiri
Entrez Gene IDs
8739 (Human)
Alternate Names
activator of apoptosis harakiri; activator of apoptosis Hrk; BCL2-interacting protein; BH3-interacting domain-containing protein 3; BID3; death protein 5; DP5; HARAKIRI; harakiri, BCL2 interacting protein (contains only BH3 domain); harakiri, BCL2-interacting protein (contains only BH3 domain); Hrk; Neuronal death protein DP5

Product Datasheets

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Citation for Human Hrk Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. BH3 profiling discriminates the anti?apoptotic status of 5?fluorouracil?resistant colon cancer cells
    Authors: K Ishikawa, Y Kawano, Y Arihara, T Kubo, K Takada, K Murase, K Miyanishi, M Kobune, J Kato
    Oncol. Rep., 2019-10-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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