Human Hrk Antibody
Human Hrk Antibody Summary
RTMWRRRARSR RAPAPGAALPC
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Human Hrk by Western Blot. Western blot shows lysates ofE. coliexpressing recombinant human Hrk-green fluorescent protein fusion. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Hrk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF851) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Hrk was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hrk
Activator of apoptosis Hrk regulates apoptosis through interaction with death-repressor proteins Bcl-2 and Bcl-X(L). The HRK protein lacks significant homology to other BCL2 family members except for an 8-amino acid region that was similar to the BCL2 homology domain-3 (BH3) motif of BIK. HRK interacts with BCL2 and BCLXL via the BH3 domain, but not with the death-promoting BCL2-related proteins BAX, BAK, or BCLXS. HRK localizes to membranes of intracellular organelles in a pattern similar to that previously reported for BCL2 and BCLXL.
Product Datasheets
Citation for Human Hrk Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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BH3 profiling discriminates the anti?apoptotic status of 5?fluorouracil?resistant colon cancer cells
Authors: K Ishikawa, Y Kawano, Y Arihara, T Kubo, K Takada, K Murase, K Miyanishi, M Kobune, J Kato
Oncol. Rep., 2019-10-15;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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