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Human ICAM-1/CD54 Antibody

Catalog #: BBA3
81 Citations 2 Reviews Datasheet / COA / SDS
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ICAM‑1/CD54 in C32 Human Cell Line.
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Citations (81)
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Human ICAM-1/CD54 Antibody Summary

Species Reactivity
Human
Specificity
This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
C32 human melanoma cell line
Flow Cytometry
0.25 µg/106 cells
Human PBMC
Immunoprecipitation
Davies, M.E., et al. (1991) Connect. Tissue Res. 26:207.
 
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC,12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
 
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

ICAM‑1/CD54 in C32 Human Cell Line. ICAM‑1/CD54 was detected in live unfixed C32 human melanoma cell line using Mouse Anti-Human ICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) at 5 µg/mL for 1 hour at 2° - 8° C. Cells were then fixed with 4% formaldehyde followed by incubation with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm.

Flow Cytometry View Larger

Detection of ICAM‑1/CD54 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # BBA3) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Western Blot View Larger

Detection of Human ICAM‑1/CD54 by Western Blot. Western blot shows lysates of C32. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ICAM‑1/CD54 at approximately 85 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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Citations for Human ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

81 Citations: Showing 1 - 10
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  1. Modeling lung endothelial dysfunction in sepsis-associated ARDS using a microphysiological system
    Authors: Liang, NW;Wilson, C;Davis, B;Wolf, I;Qyli, T;Moy, J;Beebe, DJ;Schnapp, LM;Kerr, SC;Faust, HE;
    Physiological reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Paracrinal regulation of neutrophil functions by coronaviral infection in iPSC-derived alveolar type II epithelial cells
    Authors: Chien, Y;Huang, XY;Yarmishyn, AA;Chien, CS;Liu, YH;Hsiao, YJ;Lin, YY;Lai, WY;Huang, SC;Lee, MS;Chiou, SH;Yang, YP;Chiou, GY;
    Virus research
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. ICAM-1 nanoclusters regulate hepatic epithelial cell polarity by leukocyte adhesion-independent control of apical actomyosin
    Authors: Cacho-Navas, C;López-Pujante, C;Reglero-Real, N;Colás-Algora, N;Cuervo, A;Conesa, JJ;Barroso, S;de Rivas, G;Ciordia, S;Paradela, A;D'Agostino, G;Manzo, C;Feito, J;Andrés, G;Molina-Jiménez, F;Majano, P;Correas, I;Carazo, JM;Nourshargh, S;Huch, M;Millán, J;
    eLife
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Immunoprecipitation, Immunocytochemistry
  4. Identification of new multi-substituted 1H-pyrazolo[3,4-c]pyridin-7(6H)-ones as soluble guanylyl cyclase (sGC) stimulators with vasoprotective and anti-inflammatory activities
    Authors: Kintos, DP;Salagiannis, K;Sgouros, A;Nikolaropoulos, SS;Topouzis, S;Fousteris, MA;
    Bioorganic chemistry
    Species: Rat
    Sample Types: Whole Cells
    Applications: ELISA Capture
  5. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity
    Authors: Orianne Constant, Ghizlane Maarifi, Jonathan Barthelemy, Marie-France Martin, Bachirou Tinto, Giovanni Savini et al.
    Emerging Microbes & Infections
  6. Probing cerebral malaria inflammation in 3D human brain microvessels
    Authors: Howard, C;Joof, F;Hu, R;Smith, JD;Zheng, Y;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. The tumor stroma influences immune cell distribution and recruitment in a PDAC-on-a-chip model
    Authors: Marlene Geyer, Lisa-Marie Gaul, Sabrina Luigia D`Agosto, Vincenzo Corbo, Karla Queiroz
    Frontiers in Immunology
  8. Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration
    Authors: Haley Ehlers, Arnaud Nicolas, Frederik Schavemaker, Jeroen P. M. Heijmans, Martin Bulst, Sebastiaan J. Trietsch et al.
    Frontiers in Immunology
  9. IL-33 Induces an Antiviral Signature in Mast Cells but Enhances Their Permissiveness for Human Rhinovirus Infection
    Authors: C Akoto, A Willis, CF Banas, JA Bell, D Bryant, C Blume, DE Davies, EJ Swindle
    Viruses, 2022-11-01;14(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. The impact of macrophages on endothelial cells is potentiated by cycling hypoxia: Enhanced tumor inflammation and metastasis
    Authors: Victor Delprat, Camille Huart, Olivier Feron, Fabrice Soncin, Carine Michiels
    Frontiers in Oncology
  11. A microengineered Brain-Chip to model neuroinflammation in humans
    Authors: Iosif Pediaditakis, Konstantia R. Kodella, Dimitris V. Manatakis, Christopher Y. Le, Sonalee Barthakur, Alexander Sorets et al.
    iScience
  12. GAS6/TAM signaling pathway controls MICA expression in multiple myeloma cells
    Authors: Andrea Kosta, Abdelilah Mekhloufi, Lorenzo Lucantonio, Alessandra Zingoni, Alessandra Soriani, Marco Cippitelli et al.
    Frontiers in Immunology
  13. IFNgamma-primed periodontal ligament cells regulate T-cell responses via IFNgamma-inducible mediators and ICAM-1-mediated direct cell contact
    Authors: W Singhatana, S Kitpakorns, M Toso, P Pavasant
    Royal Society open science, 2022-07-27;9(7):220056.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Plasmolipin regulates basolateral-to-apical transcytosis of ICAM-1 and leukocyte adhesion in polarized hepatic epithelial cells
    Authors: Cristina Cacho-Navas, Natalia Reglero-Real, Natalia Colás-Algora, Susana Barroso, Gema de Rivas, Kostantinos Stamatakis et al.
    Cellular and Molecular Life Sciences
  15. Endothelial cells are not productively infected by SARS‐CoV‐2
    Authors: Lilian Schimmel, Keng Yih Chew, Claudia J Stocks, Teodor E Yordanov, Patricia Essebier, Arutha Kulasinghe et al.
    Clinical & Translational Immunology
  16. Shear forces induce ICAM-1 nanoclustering on endothelial cells that impact on T-cell migration
    Authors: Izabela K. Piechocka, Sarah Keary, Alberto Sosa-Costa, Lukas Lau, Nitin Mohan, Jelena Stanisavljevic et al.
    Biophysical Journal
  17. Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype
    Authors: O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr
    PloS Pathogens, 2021-04-19;17(4):e1009522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
    Authors: E Russo, P Runge, NH Jahromi, H Naboth, A Landtwing, R Montecchi, N Leicht, MC Hunter, Y Takai, C Halin
    Cells, 2021-01-15;10(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway
    Authors: Dafeng Yang, Stefan Haemmig, Haoyang Zhou, Daniel Pérez-Cremades, Xinghui Sun, Lei Chen et al.
    eLife
  20. Human CD4+ T cell subsets differ in their abilities to cross endothelial and epithelial brain barriers in vitro
    Authors: Hideaki Nishihara, Sasha Soldati, Adrien Mossu, Maria Rosito, Henriette Rudolph, William A. Muller et al.
    Fluids and Barriers of the CNS
  21. Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis
    Authors: Masahiro Takeuchi, Paula T Vidigal, Mateus T Guerra, Melanie A Hundt, Marie E Robert, Maria Olave-Martinez et al.
    Gut
  22. Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair
    Authors: H Israelov, O Ravid, D Atrakchi, D Rand, S Elhaik, Y Bresler, R Twitto-Gre, L Omesi, S Liraz-Zalt, F Gosselet, M Schnaider, I Cooper
    J Neuroinflammation, 2020-09-09;17(1):267.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Western Blot
  23. Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier
    Authors: M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas
    MBio, 2020-08-04;11(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. hnRNPA2/B1 Ameliorates LPS-Induced Endothelial Injury through NF-&kappaB Pathway and VE-Cadherin/&beta-Catenin Signaling Modulation In Vitro
    Authors: Y Chen, D Tang, L Zhu, T Yuan, Y Jiao, H Yan, W Yu
    Mediators Inflamm., 2020-05-30;2020(0):6458791.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process
    Authors: SP Davies, GM Reynolds, AL Wilkinson, X Li, R Rose, M Leekha, YS Liu, R Gandhi, E Buckroyd, J Grove, NM Barnes, RC May, SG Hubscher, DH Adams, Y Huang, O Qureshi, Z Stamataki
    Cell Rep, 2019-11-05;29(6):1610-1620.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: IF
  26. Dual complementary liposomes inhibit triple-negative breast tumor progression and metastasis
    Authors: P Guo, J Yang, D Liu, L Huang, G Fell, J Huang, MA Moses, DT Auguste
    Sci Adv, 2019-03-20;5(3):eaav5010.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. 3D human microvessel-on-a-chip model for studying monocyte-to-endothelium adhesion under flow – application in systems toxicology
    Authors: Carine Poussin, Bart Kramer, Henriette L Lanz, Angelique Van den Heuvel, Alexandra Laurent, Thomas Olivier et al.
    ALTEX
  28. Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis
    Authors: MR Blatchley, F Hall, S Wang, HC Pruitt, S Gerecht
    Sci Adv, 2019;5(3):eaau7518.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes
    Authors: G Ecklu-Mens, RW Olsen, A Bengtsson, MF Ofori, L Hviid, ATR Jensen, Y Adams
    PLoS ONE, 2018-10-09;13(10):e0204177.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  30. Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts
    Authors: E Raschi, CB Chighizola, L Cesana, D Privitera, F Ingegnoli, C Mastaglio, PL Meroni, MO Borghi
    Arthritis Res. Ther., 2018-08-29;20(1):187.
    Species: Human
    Sample Types: Whole Cells
    Applications: Inhibition
  31. Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
    Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
    Molecular Medicine Reports, 2018-06-20;0(0):1791-3004.
    Species: Human
    Sample Types: Cell Lysate, Cell Lysates
    Applications: Western Blot
  32. Pinocembrin protects endothelial cells from oxidized LDL-induced injury
    Authors: Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li
    Cytokine, 2018-06-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Augmented reduction in colonic inflammatory markers of dextran sulfate sodium-induced colitis with a combination of 5-aminosalicylic acid and AD-lico™ from Glycyrrhiza inflata
    Authors: Jaeyoung Cho, Hyuck-Se Kweon, Sung-Oh Huh, Ali Sadra
    Animal Cells and Systems
  34. Matrix metalloproteinase-9 (MMP9) is involved in the TNF-α-induced fusion of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 cancer cells
    Authors: J Weiler, M Mohr, KS Zänker, T Dittmar
    Cell Commun. Signal, 2018-04-10;16(1):14.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Immunoprecipitation, Neutralization
  35. Inflammation-Sensitive Myosin-X Functionally Supports Leukocyte Extravasation by Cdc42-Mediated ICAM-1-Rich Endothelial Filopodia Formation
    Authors: J Kroon, A Schaefer, J van Rijsse, M Hoogenboez, F van Alphen, P Hordijk, ESG Stroes, S Strömblad, J van Rheene, JD van Buul
    J. Immunol., 2018-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bead-based Bioassay, ICC
  36. DL-3-n-butylphthalide protects endothelial cells against advanced glycation end product-induced injury by attenuating oxidative stress and inflammation responses
    Authors: Chang-Yun Liu, Zhen-Hua Zhao, Zhi-Ting Chen, Chun-Hui Che, Zhang-Yu Zou, Xiao-Min Wu et al.
    Experimental and Therapeutic Medicine
  37. ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood–brain barrier
    Authors: Ruth Lyck, Marc-André Lécuyer, Michael Abadier, Christof B Wyss, Christoph Matti, Maria Rosito et al.
    Journal of Cerebral Blood Flow & Metabolism
  38. Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro
    Authors: A Schaefer, TJ van Duijn, J Majolee, K Burridge, PL Hordijk
    J. Immunol., 2017-05-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay, ICC
  39. CD54-Mediated Interaction with Pro-inflammatory Macrophages Increases the Immunosuppressive Function of Human Mesenchymal Stromal Cells
    Authors: Nicolas Espagnolle, Adélie Balguerie, Emmanuelle Arnaud, Luc Sensebé, Audrey Varin
    Stem Cell Reports
  40. A quantitative method for screening and identifying molecular targets for nanomedicine
    Authors: P Guo, J Yang, DR Bielenberg, D Dillon, D Zurakowski, MA Moses, DT Auguste
    J Control Release, 2017-03-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. A possible role for neutrophils in allergic rhinitis revealed after cellular subclassification
    Authors: J Arebro, S Ekstedt, E Hjalmarsso, O Winqvist, S Kumlien Ge, LO Cardell
    Sci Rep, 2017-03-08;7(0):43568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells
    Authors: Minu Karthika Ganesan
    Tissue Eng Part C Methods, 2017-01-01;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: ICC
  43. A novel immune resistance mechanism of melanoma cells controlled by the ADAR1 enzyme
    Authors: Gilli Galore-Haskel, Yael Nemlich, Eyal Greenberg, Shira Ashkenazi, Motti Hakim, Orit Itzhaki et al.
    Oncotarget
  44. C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation.
    Authors: Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
    Sci Rep, 2015-03-20;5(0):9275.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  45. Role of cortactin homolog HS1 in transendothelial migration of natural killer cells.
    Authors: Mukherjee S, Kim J, Mooren O, Shahan S, Cohan M, Cooper J
    PLoS ONE, 2015-02-27;10(2):e0118153.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  46. Cycling Hypoxia Induces a Specific Amplified Inflammatory Phenotype in Endothelial Cells and Enhances Tumor-Promoting Inflammation In Vivo12
    Authors: Céline Tellier, Déborah Desmet, Laurenne Petit, Laure Finet, Carlos Graux, Martine Raes et al.
    Neoplasia
  47. Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application.
    Authors: Malara N, Trunzo V, Musolino G, Aprigliano S, Rotta G, Macrina L, Limongi T, Gratteri S, Di Fabrizio E, Renzulli A, Fini M, Mollace V
    Int J Cardiol Heart Vasc, 2015-01-09;6(0):48-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  48. Uveal melanoma cells utilize a novel route for transendothelial migration.
    Authors: Onken, Michael, Li, Jinmei, Cooper, John A
    PLoS ONE, 2014-12-15;9(12):e115472.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  49. Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier.
    Authors: Mooren O, Li J, Nawas J, Cooper J
    Mol Biol Cell, 2014-10-29;25(25):4115-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
    Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
    PLoS ONE, 2014-07-18;9(7):e102598.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  51. Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
    Authors: Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P
    PLoS ONE, 2014-04-21;9(4):e94860.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  52. Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: implications for allogeneic cell therapy of DMD.
    Authors: Noviello M, Tedesco F, Bondanza A, Tonlorenzi R, Rosaria Carbone M, Gerli M, Marktel S, Napolitano S, Cicalese M, Ciceri F, Peretti G, Cossu G, Bonini C
    Mol Ther, 2014-04-16;22(7):1342-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  53. Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.
    Authors: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K, Asashima, Makoto
    Sci Rep, 2014-04-11;4(0):4646.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  54. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
    Authors: Park, Su-Jung, Kim, Ki-Jo, Kim, Wan-Uk, Cho, Chul-Soo
    J Immunol, 2014-02-26;192(7):3003-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  55. GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.
    Authors: Lin, Yi-Wen, Huang, Chun-Yao, Chen, Yung-Hsi, Shih, Chun-Min, Tsao, Nai-Wen, Lin, Cheng-Ye, Chang, Nen-Chun, Tsai, Chien-Su, Tsai, Hsiao-Ya, Tsai, Jui-Chi, Huang, Po-Hsun, Li, Chi-Yuan, Lin, Feng-Yen
    PLoS ONE, 2013-12-23;8(12):e84731.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  56. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
    Authors: Lauridsen H, Pober J, Gonzalez A
    FASEB J, 2013-12-02;28(3):1166-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  57. Zoledronate attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of Rho/ROCK-dependent JNK and NF-kappaB pathway.
    Authors: Tsai S, Huang P, Peng Y, Chang W, Tsai H, Leu H, Chen J, Lin S
    Cardiovasc Res, 2013-12-01;100(3):501-10.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  58. Tumor-associated fibroblasts isolated from colorectal cancer tissues exhibit increased ICAM-1 expression and affinity for monocytes.
    Authors: Schellerer V, Langheinrich M, Hohenberger W, Croner R, Merkel S, Rau T, Sturzl M, Naschberger E
    Oncol Rep, 2013-11-20;31(1):255-61.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  59. Loss of p53 in stromal fibroblasts promotes epithelial cell invasion through redox-mediated ICAM1 signal
    Authors: Dunyaporn Trachootham, Gang Chen, Wan Zhang, Weiqin Lu, Hui Zhang, Jinsong Liu et al.
    Free Radical Biology and Medicine
  60. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012-09-17;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  61. Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response
    Authors: Kevin Wilhelmsen, Kailin R Mesa, Arun Prakash, Fengyun Xu, Judith Hellman
    Innate Immunity
  62. OxLDL and substrate stiffness promote neutrophil transmigration by enhanced endothelial cell contractility and ICAM-1
    Authors: Kimberly M. Stroka, Irena Levitan, Helim Aranda-Espinoza
    Journal of Biomechanics
  63. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012-06-15;287(32):26478-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell-based ELISA
  64. Endothelial cell substrate stiffness influences neutrophil transmigration via myosin light chain kinase-dependent cell contraction.
    Authors: Stroka KM, Aranda-Espinoza H
    Blood, 2011-06-07;118(6):1632-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  65. Deficiency of the NR4A Orphan Nuclear Receptor NOR1 Decreases Monocyte Adhesion and Atherosclerosis
    Authors: Yue Zhao, Deborah A. Howatt, Florence Gizard, Takashi Nomiyama, Hannes M. Findeisen, Elizabeth B. Heywood et al.
    Circulation Research
  66. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010-03-31;184(9):4880-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  67. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009-07-30;51(4):734-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC, Neutralization
  68. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
    Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P
    Am. J. Physiol. Heart Circ. Physiol., 2007-03-23;293(1):H610-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  69. Glucocorticoid-induced tumour necrosis factor receptor-related protein-mediated macrophage stimulation may induce cellular adhesion and cytokine expression in rheumatoid arthritis.
    Authors: Bae E, Kim WJ, Kang YM, Suk K, Koh EM, Cha HS, Ahn KS, Huh TL, Lee WH
    Clin. Exp. Immunol., 2007-03-15;148(3):410-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  70. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
    Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
    J. Leukoc. Biol., 2006-12-18;81(3):748-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  71. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006-07-01;54(7):2096-108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  72. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  73. Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
    Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
    Blood, 2005-11-29;107(6):2252-61.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  74. Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1.
    Authors: Domeij H, Modeer T, Quezada HC, Yucel-Lindberg T
    Biochem. Biophys. Res. Commun., 2005-11-02;338(4):1825-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  75. Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
    Authors: Tamai R, Asai Y, Ogawa T
    Infect. Immun., 2005-10-01;73(10):6290-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  76. Differential effects of orbital and laminar shear stress on endothelial cells.
    Authors: Frattini J, Kudo FA
    J. Vasc. Surg., 2005-05-01;41(5):869-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  77. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  78. CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
    Authors: Yamamoto H, Nagata M, Sakamoto Y
    Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  79. Apical topography and modulation of ICAM-1 expression on activated endothelium.
    Authors: Almenar-Queralt A, Duperray A, Miles LA, Felez J, Altieri DC
    Am. J. Pathol., 1995-11-01;147(5):1278-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  80. Circulating tumor cells from prostate cancer patients interact with E-selectin under physiologic blood flow.
    Authors: Gakhar, Gunjan, Navarro, Vicente, Jurish, Madelyn, Lee, Guang Yu, Tagawa, Scott T, Akhtar, Naveed H, Seandel, Marco, Geng, Yue, Liu, He, Bander, Neil H, Giannakakou, Paraskev, Christos, Paul J, King, Michael, Nanus, David M
    PLoS ONE, 2013-12-27;8(12):e85143.
  81. LncRNA VINAS regulates atherosclerosis by modulating NF-&kappaB and MAPK signaling
    Authors: V Simion, H Zhou, JB Pierce, D Yang, S Haemmig, Y Tesmenitsk, G Sukhova, PH Stone, P Libby, MW Feinberg
    JCI Insight, 2020-11-05;0(0):.

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Human ICAM-1/CD54 Antibody
By Anonymous on 08/12/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Endothelial cells Species: Human
Immunocytochemistry/Immunofluorescence Human ICAM-1/CD54 Antibody BBA3
Human ICAM-1/CD54 Antibody
By Michelle Chen on 04/26/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

HUVECs were stimulated with LPS (10 ng/mL)

Immunocytochemistry/Immunofluorescence Human ICAM-1/CD54 Antibody BBA3