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Human ICAM-1/CD54 Biotinylated Antibody

Catalog #: BBA9
6 Citations Datasheet / COA / SDS
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BBA9
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Citations (6)
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Human ICAM-1/CD54 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM-1 in Western blots. In this format, this antibody does not cross‑react with rhCEACAM-1, rhICAM-2, -3, -5, rmICAM-2, rrICAM-1, rmMAdCAM, or rhVCAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-I1 (11C81)
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated human umbilical vein endothelial cells (HUVEC)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ICAM‑1/CD54 Fc Chimera (Catalog # 720-IC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells, and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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Citations for Human ICAM-1/CD54 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Modified two-step emulsion solvent evaporation technique for fabricating biodegradable rod-shaped particles in the submicron size range
    Authors: H Safari, R Adili, M Holinstat, O Eniola-Ade
    J Colloid Interface Sci, 2018-02-09;518(0):174-183.
    Species: Human
    Sample Types: Microparticles, Whole Cells
    Applications: Flow Cytometry, Functional Assay
  2. Annexin A2 Limits Neutrophil Transendothelial Migration by Organizing the Spatial Distribution of ICAM-1.
    Authors: Heemskerk N, Asimuddin M, Oort C, van Rijssel J, van Buul J
    J Immunol, 2016-02-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Plasma protein corona modulates the vascular wall interaction of drug carriers in a material and donor specific manner.
    Authors: Sobczynski D, Charoenphol P, Heslinga M, Onyskiw P, Namdee K, Thompson A, Eniola-Adefeso O
    PLoS ONE, 2014-09-17;9(9):e107408.
    Applications: Bioassay
  4. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
    Authors: Arnold R, Neumann M, Konig W
    Immunology, 2007-05-01;121(1):71-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Blockade
  5. Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
    Authors: Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA
    J. Vasc. Surg., 2004-03-01;39(3):613-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling
    Authors: Niels Heemskerk, Lilian Schimmel, Chantal Oort, Jos van Rijssel, Taofei Yin, Bin Ma et al.
    Nature Communications

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