Human ICAM-2/CD102 Antibody Summary
Lys25-Gln223
Accession # CAA33630
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ICAM-2/CD102 by Western Blot ICAM-2 WT and variants co-precipitated with alpha -actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha -actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha -actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha -actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha -actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human ICAM-2/CD102 by Western Blot ICAM-2 WT and variants co-precipitated with alpha -actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha -actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha -actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha -actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha -actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-2/CD102
Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.
Product Datasheets
Citations for Human ICAM-2/CD102 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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N-glycosylation of ICAM-2 is required for ICAM-2-mediated complete suppression of metastatic potential of SK-N-AS neuroblastoma cells.
Authors: Feduska JM, Garcia PL, Brennan SB et al.
BMC Cancer
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Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process
Authors: SP Davies, GM Reynolds, AL Wilkinson, X Li, R Rose, M Leekha, YS Liu, R Gandhi, E Buckroyd, J Grove, NM Barnes, RC May, SG Hubscher, DH Adams, Y Huang, O Qureshi, Z Stamataki
Cell Rep, 2019-11-05;29(6):1610-1620.e4.
Species: Human
Sample Types: Whole Cells
Applications: IF -
ICAM-2 confers a non-metastatic phenotype in neuroblastoma cells by interaction with alpha-actinin.
Authors: Feduska J, Aller S, Garcia P, Cramer S, Council L, van Waardenburg R, Yoon K
Oncogene, 2014-04-07;34(12):1553-62.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis Rheum., 2007-06-01;56(6):1817-26.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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