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Human ICAM-2/CD102 Biotinylated Antibody

Catalog #: BAF244
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BAF244
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Human ICAM-2/CD102 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM‑2/CD102 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) ICAM-1 and rhPECAM is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ICAM‑2/CD102
Lys25-Gln223
Accession # CAA33630
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ICAM‑2/CD102 Fc Chimera (Catalog # 803-I2)
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood monocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-2/CD102

Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.

References
  1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
  2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 2
Entrez Gene IDs
3384 (Human); 15896 (Mouse)
Alternate Names
CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2

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Citation for Human ICAM-2/CD102 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Opposing roles of blood myeloid and plasmacytoid dendritic cells in HIV-1 infection of T cells: transmission facilitation versus replication inhibition.
    Authors: Groot F, van Capel TM, Kapsenberg ML, Berkhout B, de Jong EC
    Blood, 2006-05-16;108(6):1957-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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