Human ICAM-4 Antibody

Catalog #: MAB8397 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8397
MAB8397-SP

Detection of ICAM‑4 in Human Red Blood Cells by Flow Cytometry.

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Human ICAM-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAM-4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 729632

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ICAM-4
Ala23-Ala240
Accession # Q14773

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ICAM‑4 in Human Red Blood Cells by Flow Cytometry. Human red blood cells were stained with Mouse Anti-Human ICAM-4 Monoclonal Antibody (Catalog # MAB8397, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-4

ICAM-4 (Intercellular Adhesion Molecule 4), also known as Landsteiner-Wiener Glycoprotein and CD242, is a 42 kDa member of the ICAM family, Ig superfamily of proteins. It is expressed on erythrocytes and erythroblasts, and serves as a receptor for LFA-1, Mac-1, and CD11c/CD18, plus alpha 4 beta 1 and alpha-V containing integrins. ICAM-4 is suggested to bind to Mac-1 on macrophages, allowing for its phagocytosis in senescence. Mature human ICAM-4 is a 249 amino acid (aa) type I transmembrane glycoprotein. It possesses a 218 aa extracellular region (aa 23-240) that contains two C2-type Ig-like domains (aa 62-124 and 146-217), and a 10 aa C-terminal cytoplasmic tail. ICAM-4 may form 85 kDa homodimers. There are three potential isoform variants. One shows a five aa substitution for aa 233-271, a second contains a 15 aa substitution for aa 14-29, and a third possesses a 141 aa substitution for aa 132-271. Over aa 31-240, human ICAM-4 shares 71% aa identity with mouse ICAM-4.

Long Name

Intercellular Adhesion Molecule 4

Entrez Gene IDs

3386 (Human); 78369 (Mouse)

Alternate Names

CD242 antigen; CD242; ICAM4; ICAM-4; intercellular adhesion molecule 4 (Landsteiner-Wiener blood group); intercellular adhesion molecule 4 (LW blood group); intercellular adhesion molecule 4; intercellular adhesion molecule 4, Landsteiner-Wiener blood group; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; Landsteiner-Wiener blood group; LWLW blood group protein