Home / ICAT / Human ICAT Antibody

Human ICAT Antibody

Catalog #: MAB3016
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3016-100
MAB3016-SP
ICAT in Human Fibrocystic Breast Tissue.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAT Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAT in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 536017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ICAT
Met1-Gln81
Accession # Q9NSA3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry ICAT antibody in Human Fibrocystic Breast Tissue by Immunohistochemistry (IHC-P). View Larger

ICAT in Human Fibrocystic Breast Tissue. ICAT was detected in immersion fixed paraffin-embedded sections of human fibrocystic breast tissue using Mouse Anti-Human ICAT Monoclonal Antibody (Catalog # MAB3016) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAT

Human ICAT is a physiological inhibitor of the beta -Catenin/Wnt signaling pathway. It is 9 kDa in size and found in both cytosol and nucleus. The molecule contains an alpha ‑helical N-terminal region (aa 9‑54) and an acidic, extended C-terminal region (aa 55‑78). The alpha -helical region binds to beta -Catenin, displacing CBP/p300, while the acidic C-terminus interacts with the beta -Catenin N-terminus, blocking Tcf interaction. Human ICAT shares 97% aa sequence identity with mouse and bovine ICAT, respectively.

Long Name
Beta-Catenin-Interacting Protein
Entrez Gene IDs
56998 (Human)
Alternate Names
beta-catenin-interacting protein ICAT; catenin, beta interacting protein 1; CTNNBIP1; ICAT; ICATbeta-interacting protein 1; Inhibitor of beta-catenin and Tcf-4

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human ICAT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. miR-26b-5p/TCF-4 Controls the Adipogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells
    Authors: Y Luo, H Ji, Y Cao, X Ding, M Li, H Song, S Li, C WaTableng, H Wu, J Meng, H Du
    Cell Transplant, 2020-01-01;29(0):9636897209344.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human ICAT Antibody

There are currently no reviews for this product. Be the first to review Human ICAT Antibody and earn rewards!

Have you used Human ICAT Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review