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Human IFN-alpha Antibody

Catalog #: 21100-2
2 Citations Datasheet / COA / SDS
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21100-2
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Citations (2)
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Human IFN-alpha Antibody Summary

Species Reactivity
Human
Specificity
Neutralizes human interferon alpha; binds to human interferon alpha with high affinity (KA ≈ 109 M-1)
Source
Monoclonal Mouse IgG1 Clone # MMHA-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Leukocyte Interferon
Formulation
50 mM Sodium Citrate; 50 mM Sodium Phosphate, pH 7.0
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilutions should be determined by each laboratory for each application.
 
ELISA
Optimal dilutions should be determined by each laboratory for each application.
 
Neutralization
Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name
Interferon alpha
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

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Citations for Human IFN-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors
    Authors: Denarda Dangaj, Marine Bruand, Alizée J. Grimm, Catherine Ronet, David Barras, Priyanka A. Duttagupta et al.
    Cancer Cell
  2. Solid-phase PEGylation of recombinant interferon alpha-2a for site-specific modification: process performance, characterization, and in vitro bioactivity.
    Authors: Lee BK, Kwon JS, Kim HJ
    Bioconjug. Chem., 2007-10-18;18(6):1728-34.
    Species: Human
    Sample Types: Protein
    Applications: Surface Plasmon Resonance

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