Human IFN-alpha / beta R1 Antibody

Catalog #: MAB2451 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2451-100
MAB2451-SP

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Detection of Human IFN‑ alpha / beta R1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-alpha / beta R1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN-alpha / beta R1 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1056928

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
Lys28-Lys436
Accession # AAA52730

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HeLa human cervical epithelial carcinoma cell line.

Flow Cytometry

25 µg/mL

U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IFN‑ alpha / beta R1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IFN‑ alpha / beta R1 Monoclonal Antibody (Catalog # MAB2451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IFN‑ alpha / beta R1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry

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Detection of IFN‑ alpha / beta R1 in U937 human histiocytic lymphoma cell line by Flow Cytometry. U937 human histiocytic lymphoma cells were stained with Mouse Anti-Human IFN‑ alpha / beta R1 Monoclonal Antibody (Catalog # MAB2451, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R1

Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Long Name

Interferon alpha/beta Receptor 1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Alternate Names

alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1