Home / IFN-alpha/beta R1 / Human IFN-alpha / beta  R1 Antibody

Human IFN-alpha / beta  R1 Antibody

Catalog #: AF245
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF245
AF245-SP
Detection of Human IFN‑ alpha / beta  R1 by Western Blot.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-alpha / beta  R1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN‑ alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IFN‑ alpha / beta  RI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta  R1
Lys28-Lys436
Accession # AAA52730
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1-1 µg/mL
Recombinant Human IFN-alpha / beta  R1, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line
Flow Cytometry
2.5 µg/106 cells
U937 human histiocytic lymphoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human IFN‑ alpha / beta  R1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFN‑ alpha / beta  R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IFN‑ alpha / beta  R1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R1

Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Long Name
Interferon alpha/beta Receptor 1
Entrez Gene IDs
3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)
Alternate Names
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1

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Product Specific Notices

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source. For research use only. Not for resale. Not for diagnostic, therapeutic or clinical purposes.

Citations for Human IFN-alpha / beta  R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants
    Authors: Y Han, L Tan, T Zhou, L Yang, L Carrau, LA Lacko, M Saeed, J Zhu, Z Zhao, BE Nilsson-Pa, FT Lira Neto, C Cahir, AM Giani, JC Chai, Y Li, X Dong, D Moroziewic, NYSCF Glob, D Paull, T Zhang, S Koo, C Tan, R Danziger, Q Ba, L Feng, Z Chen, A Zhong, GJ Wise, JZ Xiang, H Wang, RE Schwartz, BR tenOever, SA Noggle, CM Rice, Q Qi, T Evans, S Chen
    Cell Stem Cell, 2022-10-06;29(10):1475-1490.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Trisomy 21 consistently activates the interferon response
    Elife, 2016-07-29;5(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Chronic exposure to type-I IFN under lymphopenic conditions alters CD4 T cell homeostasis.
    Authors: Le Saout C, Hasley R, Imamichi H, Tcheung L, Hu Z, Luckey M, Park J, Durum S, Smith M, Rupert A, Sneller M, Lane H, Catalfamo M
    PLoS Pathog, 2014-03-06;10(3):e1003976.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Murine plasmacytoid dendritic cells produce IFN-gamma upon IL-4 stimulation.
    Authors: Suto A, Nakajima H, Tokumasa N, Takatori H, Kagami S, Suzuki K, Iwamoto I
    J. Immunol., 2005-11-01;175(9):5681-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Type I and type II interferons delay human neutrophil apoptosis via activation of STAT3 and up-regulation of cellular inhibitor of apoptosis 2.
    Authors: Sakamoto E, Hato F, Kato T, Sakamoto C, Akahori M, Hino M, Kitagawa S
    J. Leukoc. Biol., 2005-04-21;78(1):301-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Blocking of the alpha interferon-induced Jak-Stat signaling pathway by Japanese encephalitis virus infection.
    Authors: Lin RJ, Liao CL, Lin E, Lin YL
    J. Virol., 2004-09-01;78(17):9285-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Autocrine type I IFN and contact with endothelium promote the presentation of influenza A virus by monocyte-derived APC.
    Authors: Qu C, Moran TM, Randolph GJ
    J. Immunol., 2003-01-15;170(2):1010-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Early IFN-alpha signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19
    Authors: Benjamin Krämer, Rainer Knoll, Lorenzo Bonaguro, Michael ToVinh, Jan Raabe, Rosario Astaburuaga-García et al.
    Immunity
  9. Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity
    Authors: Sabrina Rueschenbaum, Chengcong Cai, Matthias Schmidt, Katharina Schwarzkopf, Ulf Dittmer, Stefan Zeuzem et al.
    Frontiers in Immunology

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