Human IFN-epsilon Antibody

Catalog #: MAB91472 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB91472-100
MAB91472-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-epsilon Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN-epsilon in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 995728

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IFN-epsilon
Leu22-Arg208
Accession # Q86WN2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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IFN-epsilon in HeLa Human Cell Line. IFN-epsilon was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human IFN-epsilon Monoclonal Antibody (Catalog # MAB91472) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-epsilon

Interferon-epsilon (IFNe) is a type I interferon with expression limited to the brain, lung, intestine and reproductive tract. It has been proposed that unlike other type I interferons, IFNe plays a role in mucosal immunity and protects against pathogens including chlamidia, HIV and herpes simplex virus 2. Its expression is upregulated by TNF alpha. Unlike other type-1 interferons, IFNe is also hormonally regulated, and mouse and human IFNe have been shown to have a binding site in their promoters for progesterone receptor. Epithelial cells isolated from uterine endometrium have higher IFNee expression in the proliferative phase, when estrogen levels are highest. IFNe is the only IFN isoform constituitively expressed in HeLa cells.

Long Name

Interferon epsilon

Entrez Gene IDs

338376 (Human); 230405 (Mouse); 298225 (Rat)

Alternate Names

IFNE; IFN-E; IFNE1PRO655; IFNepsilon; IFN-epsilon; IFNT1; Ifn-tau-11; INFE1; interferon epsilon 1; interferon epsilon; Interferon epsilon-1; interferon tau-1; interferon, epsilon; interferon-epsilon; MGC119018; MGC119020