Human IFN-gamma Antibody Summary
Gln24-Gln166
Accession # AAP20098.1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
IFN‑ gamma in Human PBMCs. IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IFN-gamma Monoclonal Antibody (Catalog # MAB285) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight were stained with either (A) Mouse Anti-Human IFN- gamma Monoclonal Antibody (Catalog # MAB285) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN-gamma Monoclonal Antibody (Catalog # MAB285). The ND50 is typically 0.02-0.06 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma
Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
Product Datasheets
Citations for Human IFN-gamma Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Clinical Use of Propranolol Reduces Biomarkers of Proliferation in Gastric Cancer
Authors: Hu Q, Liao P, Li W et al.
Frontiers in Oncology
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Interferon subverts an AHR-JUN axis to promote CXCL13+ T cells in lupus
Authors: Law, C;Wacleche, VS;Cao, Y;Pillai, A;Sowerby, J;Hancock, B;Horisberger, A;Bracero, S;Skidanova, V;Li, Z;Adejoorin, I;Dillon, E;Benque, IJ;Nunez, DP;Simmons, DP;Keegan, J;Chen, L;Baker, T;Brohawn, PZ;Al-Mossawi, H;Hao, LY;Jones, B;Rao, N;Qu, Y;Alves, SE;Accelerating Medicines Partnership: RA/SLE Network, ;Jonsson, AH;Shaw, KS;Vleugels, RA;Massarotti, E;Costenbader, KH;Brenner, MB;Lederer, JA;Hultquist, JF;Choi, J;Rao, DA;
Nature
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Targeting high circDNA2v levels in colorectal cancer induces cellular senescence and elicits an anti-tumor secretome
Authors: Wu, S;Dai, X;Xia, Y;Zhao, Q;Zhao, H;Shi, Z;Yin, X;Liu, X;Zhang, A;Yao, Z;Zhang, H;Li, Q;Thorne, RF;Zhang, S;Sheng, W;Hu, W;Gu, H;
Cell reports
Species: Human
Sample Types: Transduced Whole Cells
Applications: Neutralization -
Dissecting human population variation in single-cell responses to SARS-CoV-2
Authors: Aquino, Y;Bisiaux, A;Li, Z;O'Neill, M;Mendoza-Revilla, J;Merkling, SH;Kerner, G;Hasan, M;Libri, V;Bondet, V;Smith, N;de Cevins, C;Ménager, M;Luca, F;Pique-Regi, R;Barba-Spaeth, G;Pietropaoli, S;Schwartz, O;Leroux-Roels, G;Lee, CK;Leung, K;Wu, JT;Peiris, M;Bruzzone, R;Abel, L;Casanova, JL;Valkenburg, SA;Duffy, D;Patin, E;Rotival, M;Quintana-Murci, L;
Nature
Species: Human
Sample Types: Cell Culture Superantes
Applications: Luminex (Detection) -
A bispecific ?? T-cell engager targeting EGFR activates a potent V?9V?2 T cell-mediated immune response against EGFR-expressing tumors
Authors: King, LA;Toffoli, EC;Veth, M;Iglesias-Guimarais, V;Slot, MC;Amsen, D;van de Ven, R;Derks, S;Fransen, MF;Tuynman, JB;Riedl, T;Roovers, RC;Adang, AEP;Ruben, JM;Parren, PWHI;de Gruijl, TD;van der Vliet, HJ;
Cancer immunology research
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata
Authors: R Laufer Bri, A Keren, M Bertolini, Y Ullmann, R Paus, A Gilhar
Elife, 2023-03-17;12(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor
Authors: AKO Rode, TB Buus, V Mraz, FAH Al-Jaberi, DV Lopez, SL Ford, S Hennen, IP Eliasen, IV Klewe, L Gharehdagh, A Dragan, MM Rosenkilde, A Woetmann, L Skov, N Ødum, CM Bonefeld, M Kongsbak-W, C Geisler
Cells, 2022-08-19;11(16):.
Species: Human
Sample Types: Whole Cells
Applications: Stimulation -
Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip
Authors: Girija Goyal, Pranav Prabhala, Gautam Mahajan, Bruce Bausk, Tal Gilboa, Liangxia Xie et al.
Advanced Science
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IFN‐ gamma facilitates liver fibrogenesis by CD161+CD4+ T cells through a regenerative IL‐23/IL‐17 axis in chronic hepatitis B virus infection
Authors: Jing Li, Lisha Cheng, Haoyu Jia, Chun Liu, Siqi Wang, Yun Liu et al.
Clinical & Translational Immunology
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Distinct systemic and mucosal immune responses during acute SARS-CoV-2 infection
Authors: N Smith, P Goncalves, B Charbit, L Grzelak, M Beretta, C Planchais, T Bruel, V Rouilly, V Bondet, J Hadjadj, N Yatim, H Pere, SH Merkling, A Ghozlane, S Kernéis, F Rieux-Lauc, B Terrier, O Schwartz, H Mouquet, D Duffy, JP Di Santo
Nature Immunology, 2021-09-01;0(0):.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection -
Expansion of Monocytic Myeloid-Derived Suppressor Cells in Patients Under Hemodialysis Might Lead to Cardiovascular and Cerebrovascular Events
Authors: Yan-Fang Xing, Jia-Rong Cai, Jun-Jian Qin, Wen-Ying Zhou, Can-Ming Li, Xing Li
Frontiers in Immunology
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Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours
Authors: Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi et al.
Transplantation
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Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway
Authors: F Ma, H Hao, X Gao, Y Cai, J Zhou, P Liang, J Lv, Q He, C Shi, D Hu, B Chen, L Zhu, X Xiao, S Li
Theranostics, 2020-06-19;10(17):7730-7746.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
In the Absence of a TCR Signal IL-2/IL-12/18-Stimulated gammadelta T Cells Demonstrate Potent Anti-Tumoral Function Through Direct Killing and Senescence Induction in Cancer Cells
Authors: K Schilbach, C Welker, N Krickeberg, C Kai beta er, S Schleicher, H Hashimoto
Cancers (Basel), 2020-01-04;12(1):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Single-cell transcriptomics of human T cells reveals tissue and activation signatures in health and disease
Authors: PA Szabo, HM Levitin, M Miron, ME Snyder, T Senda, J Yuan, YL Cheng, EC Bush, P Dogra, P Thapa, DL Farber, PA Sims
Nat Commun, 2019-10-17;10(1):4706.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Early Detection of Peripheral Blood Cell Signature in Children Developing beta -Cell Autoimmunity at a Young Age
Authors: Henna Kallionpää, Juhi Somani, Soile Tuomela, Ubaid Ullah, Rafael de Albuquerque, Tapio Lönnberg et al.
Diabetes
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TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2
Authors: Paramita Baruah, Jessica Bullenkamp, Philip O. G. Wilson, Michael Lee, Juan Carlos Kaski, Ingrid E. Dumitriu
Frontiers in Immunology
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Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock
Authors: Amaro Nunes Duarte-Neto, Julio Croda, Carla Pagliari, Francisco Garcia Soriano, Antonio Carlos Nicodemo, Maria Irma Seixas Duarte
Frontiers in Immunology
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Alloreactive fetal T cells promote uterine contractility in preterm labor via IFN-gamma and TNF-alpha
Authors: Michela Frascoli, Lacy Coniglio, Russell Witt, Cerine Jeanty, Shannon Fleck-Derderian, Dana E. Myers et al.
Science Translational Medicine
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DDIT4 and Associated lncDDIT4 Modulate Th17 Differentiation through the DDIT4/TSC/mTOR Pathway
Authors: F Zhang, G Liu, D Li, C Wei, J Hao
J. Immunol., 2018-01-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2
Authors: Y Liu, L Qu, Y Liu, B Roizman, GG Zhou
Proc. Natl. Acad. Sci. U.S.A., 2017-07-31;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Bilirubin suppresses Th17 immunity in colitis by upregulating CD39
Authors: MS Longhi, M Vuerich, A Kalbasi, JE Kenison, A Yeste, E Csizmadia, B Vaughn, L Feldbrugge, S Mitsuhashi, B Wegiel, L Otterbein, A Moss, FJ Quintana, SC Robson
JCI Insight, 2017-05-04;2(9):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Innate Invariant NKT Cell Recognition of HIV-1-Infected Dendritic Cells Is an Early Detection Mechanism Targeted by Viral Immune Evasion
J Immunol, 2016-08-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
Modulation of Stat-1 in Human Macrophages Infected with Different Species of Intracellular Pathogenic Bacteria
J Immunol Res, 2016-06-29;2016(0):5086928.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Production of Proinflammatory Cytokines by Monocytes in Liver-Transplanted Recipients with De Novo Autoimmune Hepatitis Is Enhanced and Induces TH1-like Regulatory T Cells
Authors: AS Arterbery, A Osafo-Addo, Y Avitzur, M Ciarleglio, Y Deng, SJ Lobritto, M Martinez, DA Hafler, M Kleinewiet, UD Ekong
J. Immunol., 2016-04-18;196(10):4040-51.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes
Authors: Fudi Zhong, Nan Zhou, Kang Wu, Yubiao Guo, Weiping Tan, Hong Zhang et al.
Nucleic Acids Research
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The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil
Nat Immunol, 2015-01-26;16(3):318-25.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
PLoS ONE, 2014-05-02;9(5):e96695.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Maintenance of Th1 hepatitis C virus (HCV)-specific responses in individuals with acute HCV who achieve sustained virological clearance after treatment
Authors: Jacqueline K Flynn, Gregory J Dore, Margaret Hellard, Barbara Yeung, William D Rawlinson, Peter A White et al.
Journal of Gastroenterology and Hepatology
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Potential Limitations of the NSG Humanized Mouse as a Model System to Optimize Engineered Human T cell Therapy for Cancer
Authors: Erik M. Alcantar-Orozco, Hannah Gornall, Vania Baldan, Robert E. Hawkins, David E. Gilham
Human Gene Therapy Methods
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Pathological and ultrastructural analysis of surgical lung biopsies in patients with swine-origin influenza type A/H1N1 and acute respiratory failure
Authors: Vera Luiza Capelozzi, Edwin Roger Parra, Manoel Ximenes, Ricardo Helbert Bammann, Carmen Silvia Valente Barbas, Marid Irmd Seixas Duarte
Clinics (Sao Paulo)
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The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation.
Authors: Chang HC, Sehra S, Goswami R, Yao W, Yu Q, Stritesky GL, Jabeen R, McKinley C, Ahyi AN, Han L, Nguyen ET, Robertson MJ, Perumal NB, Tepper RS, Nutt SL, Kaplan MH
Nat. Immunol., 2010-05-02;11(6):527-34.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Plasma from systemic lupus patients compromises cholesterol homeostasis: a potential mechanism linking autoimmunity to atherosclerotic cardiovascular disease
Authors: Allison B. Reiss, Kamran Anwar, Joan T. Merrill, Edwin S. L. Chan, Nahel W. Awadallah, Bruce N. Cronstein et al.
Rheumatology International
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Tissue and serum immune response in chronic hepatitis C with mild histological lesions
Authors: A T R-Viso, M I S Duarte, C Pagliari, E R Fernandes, R A Brasil, G Benard et al.
Memórias do Instituto Oswaldo Cruz
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Impaired development of human Th1 cells in patients with deficient expression of STAT4.
Authors: Chang HC, Han L, Goswami R, Nguyen ET, Pelloso D, Robertson MJ, Kaplan MH
Blood, 2009-04-09;113(23):5887-90.
Species: Human
Sample Types: Whole Cells
Applications: Differentiation, Differentiation -
In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
J. Clin. Invest., 2007-12-01;117(12):3868-3878.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
Exp. Hematol., 2006-10-01;34(10):1312-22.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Authors: Ebert EC, Mehta V
Cell. Immunol., 2006-08-17;241(1):7-13.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Peripheral expansion of circulating T-helper 1 cells predicts coronary endothelial dysfunction after cardiac transplantation.
Authors: Methe H, Wiegand D, Welsch U, Meiser B, Nabauer M, Koglin J
J. Heart Lung Transplant., 2005-07-01;24(7):833-40.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
J. Immunol., 2004-07-15;173(2):1463-76.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
J. Immunol., 2003-11-15;171(10):5034-41.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment.
Authors: Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y
Blood, 2003-07-31;102(12):4084-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cutting edge: immune cells as sources and targets of the IL-10 family members?
Authors: Wolk K, Kunz S, Asadullah K, Sabat R
J. Immunol., 2002-06-01;168(11):5397-402.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis.
Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY
J. Immunol., 2002-01-01;168(1):505-10.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CD1d-mediated Recognition of an alpha -Galactosylceramide by Natural Killer T Cells Is Highly Conserved through Mammalian Evolution
Authors: Laurent Brossay, Mariacristina Chioda, Nicolas Burdin, Yasuhiko Koezuka, Giulia Casorati, Paolo Dellabona et al.
J. Exp. Med
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